Sebatini A Maria, Jain Manisha, Radha P, Kiruthika S, Tamilarasan Krishnamurthi
Department of Biotechnology, School of Bioengineering, SRM University, Chennai, Tamilnadu, 603203, India.
Department of Chemical Engineering, School of Bioengineering, SRM University, Chennai, Tamilnadu, 603203, India.
3 Biotech. 2016 Dec;6(2):184. doi: 10.1007/s13205-016-0501-z. Epub 2016 Aug 29.
Sugar fatty acid esters are practical importance and have a variety of applications that include surfactants and as an emulsifying agent. In this study, we report glucose stearate synthesis using lipase-FeO nanoparticles catalyst. The influence of various reaction factors, such as silica gel concentration, molar ratio of sugar/acid, reaction temperature and speed of agitation on esterification by immobilized enzyme was analyzed. The glucose stearate esterification degree of 87.2 % was obtained under the optimized condition: 1:2 molar ratio of glucose/stearic acid, 2 % (w/v) of silica gel at 120 rpm and 40 °C. Glucose esters were characterized according to their surfactant activity like emulsification index, oil displacement activity and antimicrobial activity. The results indicated glucose stearate acts as biosurfactant, with emulsification index of 66 % in mustard oil and oil displacement activity of 19.64 cm.
糖脂肪酸酯具有实际重要性，并有多种应用，包括用作表面活性剂和乳化剂。在本研究中，我们报道了使用脂肪酶 - 氧化铁纳米颗粒催化剂合成葡萄糖硬脂酸酯。分析了各种反应因素，如硅胶浓度、糖/酸摩尔比、反应温度和搅拌速度对固定化酶酯化反应的影响。在优化条件下：葡萄糖/硬脂酸摩尔比为1:2、硅胶2%（w/v）、转速120 rpm和温度40°C，获得了87.2%的葡萄糖硬脂酸酯化度。根据其表面活性剂活性，如乳化指数、驱油活性和抗菌活性对葡萄糖酯进行了表征。结果表明，葡萄糖硬脂酸酯作为生物表面活性剂，在芥子油中的乳化指数为66%，驱油活性为19.64 cm。
