Institute of Molecular and Cellular Biology, Faculty of Biological Sciences, Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds LS2 6JP, UK.
Biochem Biophys Res Commun. 2012 Mar 9;419(2):137-41. doi: 10.1016/j.bbrc.2011.11.126. Epub 2012 Jan 28.
The arterivirus nucleocapsid (N) protein is a multifunctional protein that binds viral RNA for encapsidation and has potential roles in host cell processes. This study characterised the N protein from a highly virulent North American strain of porcine reproductive and respiratory syndrome virus (PRRSV). The association with viral RNA was mapped to defined motifs on the N protein. The results indicated that disulphide bridge formation played a key role in RNA binding, offering an explanation why infectious virus cannot be rescued if cysteine residues are mutated, and that multiple sites may promote RNA binding.
动脉炎病毒核衣壳（N）蛋白是一种多功能蛋白，可结合病毒 RNA 进行包裹，并在宿主细胞过程中具有潜在作用。本研究对来自北美高致病性猪繁殖与呼吸综合征病毒（PRRSV）的 N 蛋白进行了特征描述。N 蛋白与病毒 RNA 的结合被定位到 N 蛋白上的特定基序。结果表明，二硫键的形成在 RNA 结合中起着关键作用，这解释了为什么如果半胱氨酸残基发生突变，就无法拯救感染性病毒，并且多个位点可能促进 RNA 结合。
