用于诊断酶联免疫吸附测定（ELISA）以检测病毒感染的重组病毒蛋白。
Recombinant viral proteins for use in diagnostic ELISAs to detect virus infection.
作者信息
Spencer Kelly-Anne, Osorio Fernando A, Hiscox Julian A
机构信息
Institute of Molecular and Cellular Biology, Faculty of Biological Sciences, University of Leeds, UK.
出版信息
Vaccine. 2007 Jul 26;25(30):5653-9. doi: 10.1016/j.vaccine.2007.02.053. Epub 2007 Mar 6.
Abstract
ELISAs provide a valuable tool in the detection and diagnosis of virus infection. The ability to produce recombinant viral proteins will ensure that future ELISAs are safe, specific and rapid. This latter point being the most crucial advantage in that even if a virus cannot be cultured, provided gene sequence is available, it is possible to rapidly respond to emerging viruses and new viral strains of existing pathogens. Indeed, ELISAs based on peptides (corresponding to epitopes) also hold great promise, as in this case no cloning or expression of a recombinant protein is required. Both recombinant protein and peptide based systems lend themselves to large scale production and purification. These approaches can also be used to distinguish recombinant vaccines from parental or wild type viruses.
摘要
酶联免疫吸附测定（ELISA）为病毒感染的检测与诊断提供了一种有价值的工具。生产重组病毒蛋白的能力将确保未来的ELISA检测安全、特异且快速。后一点是最为关键的优势，因为即便一种病毒无法培养，但只要有基因序列，就有可能对新出现的病毒以及现有病原体的新病毒株做出快速反应。实际上，基于肽段（对应于表位）的ELISA也很有前景，因为在这种情况下无需克隆或表达重组蛋白。基于重组蛋白和肽段的系统都适合大规模生产与纯化。这些方法还可用于区分重组疫苗与亲本病毒或野生型病毒。
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Methods Mol Biol. 2007;363:1-19. doi: 10.1007/978-1-59745-209-0_1.
2
A structural-energetic basis for B-cell epitope prediction.B细胞表位预测的结构-能量基础
Protein Pept Lett. 2006;13(7):743-51. doi: 10.2174/092986606777790502.
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Vet Microbiol. 2006 Dec 20;118(3-4):201-11. doi: 10.1016/j.vetmic.2006.07.023. Epub 2006 Aug 2.
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