Zhang Fan-Tao, Fang Jun, Sun Chang-Hui, Li Run-Bao, Luo Xiang-Dong, Xie Jian-Kun, Deng Xiao-Jian, Chu Cheng-Cai
Sichuan Agricultural University, Chengdu, China.
Yi Chuan. 2012 Jan;34(1):79-86. doi: 10.3724/sp.j.1005.2012.00079.
Plant height is one of the most important agronomic traits, which determines grain yield. By a largescale screening of our mutant population, we identified a dwarf with twisty leaf mutant (dwarf and twist leaf 1, dtl1). Besides dwarf with twisty leaf, dtl1 also showed reduced tiller number and sterile phenotypes. Based on the internode length of dtl1, this mutant belongs to the nl type of dwarfing phenotype. Physiological assay with two phytohormones, gibberellin (GA), and brassinosteroid (BR), suggested that dtl1 was neither deficient nor insensitive to GA and BR. Genetic analysis showed that the phenotype of dtl1 was controlled by a single recessive gene. Using F2 population derived from a cross between dtl1 and an indica cultivar Taichung Native 1, the DTL1 gene was narrowed down to a 70.4 kb between two SSR markers, RM25923 and RM6673, on the long arm of chromosome 10, and co-segregated with InDel marker Z10-29, where thirteen open reading frames were predicted without known gene involved in controlling plant height. Thus, the DTL1 gene might be a novel gene which is related to plant height in rice.
株高是最重要的农艺性状之一，它决定着谷物产量。通过对我们的突变体群体进行大规模筛选，我们鉴定出了一个叶片扭曲的矮化突变体（矮化扭曲叶1，dtl1）。除了叶片扭曲的矮化表型外，dtl1还表现出分蘖数减少和不育的表型。根据dtl1的节间长度，该突变体属于nl型矮化表型。用两种植物激素赤霉素（GA）和油菜素内酯（BR）进行的生理分析表明，dtl1对GA和BR既不缺乏也不敏感。遗传分析表明，dtl1的表型受单个隐性基因控制。利用dtl1与籼稻品种台中本地1杂交产生的F2群体，将DTL1基因定位到第10号染色体长臂上两个SSR标记RM25923和RM6673之间的70.4 kb区间内，并与InDel标记Z10-29共分离，在该区间预测有13个开放阅读框，但没有已知参与控制株高的基因。因此，DTL1基因可能是一个与水稻株高相关的新基因。
