State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Key Laboratory of Plant Functional Genomics and Biotechnology of Guangdong Provincial Higher Education Institutions, College of Life Sciences, South China Agricultural University, Guangzhou, 510642, China.
Theor Appl Genet. 2014 Jan;127(1):241-50. doi: 10.1007/s00122-013-2213-7. Epub 2013 Oct 25.
Plant height is an important agronomic trait for crop architecture and yield. Most known factors determining plant height function in gibberellin or brassinosteroid biosynthesis or signal transduction. Here, we report a japonica rice (Oryza sativa ssp. japonica) dominant dwarf mutant, Photoperiod-sensitive dwarf 1 (Psd1). The Psd1 mutant showed impaired cell division and elongation, and a severe dwarf phenotype under long-day conditions, but nearly normal growth in short-day. The plant height of Psd1 mutant could not be rescued by gibberellin or brassinosteroid treatment. Genetic analysis with R1 and F2 populations determined that Psd1 phenotype was controlled by a single dominant locus. Linkage analysis with 101 tall F2 plants grown in a long-day season, which were derived from a cross between Psd1 and an indica cultivar, located Psd1 locus on chromosome 1. Further fine-mapping with 1017 tall F2 plants determined this locus on an 11.5-kb region. Sequencing analysis of this region detected a mutation site in a gene encoding a putative lipid transfer protein; the mutation produces a truncated C-terminus of the protein. This study establishes the genetic foundation for understanding the molecular mechanisms regulating plant cell division and elongation mediated by interaction between genetic and environmental factors.
株高是作物结构和产量的一个重要农艺性状。大多数已知的决定株高的因素在赤霉素或油菜素内酯的生物合成或信号转导中起作用。在这里，我们报道了一个粳稻（Oryza sativa ssp. japonica）显性矮秆突变体，光周期敏感矮秆 1（Psd1）。在长日照条件下，Psd1 突变体表现出细胞分裂和伸长受损，以及严重的矮秆表型，但在短日照下几乎正常生长。赤霉素或油菜素内酯处理不能挽救 Psd1 突变体的株高。用 R1 和 F2 群体进行的遗传分析表明，Psd1 表型由一个单一的显性基因控制。用 101 株在长日照季节生长的高秆 F2 植物进行连锁分析，这些植物是由 Psd1 和一个籼稻品种杂交产生的，将 Psd1 基因定位在第 1 号染色体上。用 1017 株高秆 F2 植物进行精细定位，将该基因定位于 11.5kb 的区域。对该区域的测序分析检测到一个编码假定脂质转移蛋白的基因中的突变位点；该突变产生了该蛋白 C 末端的截断。这项研究为理解遗传和环境因素相互作用介导的植物细胞分裂和伸长的分子机制奠定了遗传基础。
