Stenger D C, Carbonaro D, Duffus J E
United States Department of Agriculture, Agricultural Research Service, Salinas, California 93905.
J Gen Virol. 1990 Oct;71 ( Pt 10):2211-5. doi: 10.1099/0022-1317-71-10-2211.
Full-length infectious DNA clones were constructed for four distinct phenotypic variants of beet curly top virus (BCTV). Southern hybridization assays indicated that each cloned BCTV genome shared sequence homology with pBCT-028, a full-length infectious DNA clone of a California isolate of BCTV previously characterized by others. Restriction endonuclease maps of the cloned BCTV genomes were distinct from one another. Infectivity assays determined that plasmids containing tandem repeats of BCTV genomes were generally more infectious than excised linear DNA inserts. Progeny virus, derived from plants inoculated with cloned DNAs, differed in their ability to infect sugarbeet, Beta vulgaris L., and the severity of symptoms produced in B. vulgaris and other experimental hosts.
构建了甜菜曲顶病毒（BCTV）四种不同表型变体的全长感染性DNA克隆。Southern杂交分析表明，每个克隆的BCTV基因组与pBCT - 028具有序列同源性，pBCT - 028是先前由其他人鉴定的加利福尼亚州BCTV分离株的全长感染性DNA克隆。克隆的BCTV基因组的限制性内切酶图谱彼此不同。感染性测定确定，含有BCTV基因组串联重复序列的质粒通常比切除的线性DNA插入片段更具感染性。从接种克隆DNA的植物中获得的子代病毒在感染甜菜（Beta vulgaris L.）的能力以及在甜菜和其他实验宿主中产生的症状严重程度方面存在差异。
