Briddon R W, Watts J, Markham P G, Stanley J
Department of Virus Research, John Innes Institute, AFRC Institute of Plant Science Research, Norwich, United Kingdom.
Virology. 1989 Oct;172(2):628-33. doi: 10.1016/0042-6822(89)90205-5.
We have applied the procedure of Agrobacterium-mediated inoculation to develop a simple, efficient, and reproducible assay for the infectivity of the leafhopper-transmitted geminivirus, beet curly top virus (BCTV). This assay system was used to show that a coat protein mutant of BCTV is not infectious, but could be complemented by coagroinoculation with a second mutant bearing a lethal mutation in the complementary-sense open reading frame, C1. Furthermore, the coat protein mutant retained the ability to replicate and to produce both ssDNA and dsDNA when electroporated into Nicotiana tabacum protoplasts. We conclude that the coat protein of BCTV is essential for spread of the virus. The results are discussed in the light of results with coat protein mutants of other geminiviruses.
我们已应用农杆菌介导接种程序，开发出一种简单、高效且可重复的方法，用于检测叶蝉传播的双生病毒——甜菜曲顶病毒（BCTV）的感染性。该检测系统用于表明，BCTV的一种外壳蛋白突变体没有感染性，但与在互补链开放阅读框C1中带有致死突变的第二个突变体共接种时，可以得到互补。此外，当将该外壳蛋白突变体电穿孔导入烟草原生质体时，它保留了复制以及产生单链DNA和双链DNA的能力。我们得出结论，BCTV的外壳蛋白对于病毒传播至关重要。结合其他双生病毒外壳蛋白突变体的研究结果对这些结果进行了讨论。
