利用抑制性消减杂交技术鉴定与变形链球菌相关的 pheA 基因。
Identification of a pheA gene associated with Streptococcus mitis by using suppression subtractive hybridization.
机构信息
Department of Microbiology, Chung-Ang University College of Medicine, Seoul, Republic of Korea.
出版信息
Appl Environ Microbiol. 2012 Apr;78(8):3004-9. doi: 10.1128/AEM.07510-11. Epub 2012 Feb 3.
Abstract
We performed suppression subtractive hybridization to identify genomic differences between Streptococcus mitis and Streptococcus pneumoniae. Based on the pheA gene, a primer set specific to S. mitis detection was found in 18 out of 103 S. mitis-specific clones. Our findings would be useful for discrimination of S. mitis from other closely related cocci in the oral environment.
摘要
我们进行抑制性消减杂交来鉴定酿脓链球菌和肺炎链球菌之间的基因组差异。基于 pheA 基因，在 103 个酿脓链球菌特异性克隆中有 18 个克隆中发现了一组特异性检测酿脓链球菌的引物。我们的研究结果将有助于区分口腔环境中其他密切相关的球菌中的酿脓链球菌。
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