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利用抑制性消减杂交技术鉴定与变形链球菌相关的 pheA 基因。

Identification of a pheA gene associated with Streptococcus mitis by using suppression subtractive hybridization.

机构信息

Department of Microbiology, Chung-Ang University College of Medicine, Seoul, Republic of Korea.

出版信息

Appl Environ Microbiol. 2012 Apr;78(8):3004-9. doi: 10.1128/AEM.07510-11. Epub 2012 Feb 3.

DOI:10.1128/AEM.07510-11
PMID:22307284
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3318815/
Abstract

We performed suppression subtractive hybridization to identify genomic differences between Streptococcus mitis and Streptococcus pneumoniae. Based on the pheA gene, a primer set specific to S. mitis detection was found in 18 out of 103 S. mitis-specific clones. Our findings would be useful for discrimination of S. mitis from other closely related cocci in the oral environment.

摘要

我们进行抑制性消减杂交来鉴定酿脓链球菌和肺炎链球菌之间的基因组差异。基于 pheA 基因,在 103 个酿脓链球菌特异性克隆中有 18 个克隆中发现了一组特异性检测酿脓链球菌的引物。我们的研究结果将有助于区分口腔环境中其他密切相关的球菌中的酿脓链球菌。

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Gut. 2012 Apr;61(4):582-8. doi: 10.1136/gutjnl-2011-300784. Epub 2011 Oct 12.
2
The rgg gene is a specific marker for Streptococcus oralis.rgg 基因是口腔链球菌的特定标志物。
J Dent Res. 2010 Nov;89(11):1299-303. doi: 10.1177/0022034510378426. Epub 2010 Aug 25.
3
Real-time PCR assays for the detection and quantification of Streptococcus pneumoniae.用于检测和定量肺炎链球菌的实时 PCR 检测法。
FEMS Microbiol Lett. 2010 Sep 1;310(1):48-53. doi: 10.1111/j.1574-6968.2010.02044.x. Epub 2010 Jun 18.
4
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5
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Rapid identification of bacteria in positive blood culture broths by matrix-assisted laser desorption ionization-time of flight mass spectrometry.基质辅助激光解吸电离飞行时间质谱法快速鉴定阳性血培养肉汤中的细菌。
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