Department of Microbiology & Research Institute for Translational System Biomics, Chung-Ang University College of Medicine, 221 Heukseok-dong, Dongjak-ku, Seoul 156-756, Republic of Korea.
J Dent Res. 2010 Nov;89(11):1299-303. doi: 10.1177/0022034510378426. Epub 2010 Aug 25.
Although the pathogenesis of Streptococcus oralis may be different from that of other viridans group streptococci, S. oralis shares a high degree of DNA sequence similarity with these streptococci. As a result, discrimination of S. oralis from its close relatives has long been considered difficult. This study was conducted to find specific genes that allow for the in vitro identification of S. oralis, but not other oral commensals. Four hundred ninety S. oralis clones obtained by suppressive subtractive hybridization were used for Southern hybridization, and positive clones were sequenced. Of 5 S. oralis-specific clones, newly designed primer sets based on the glucosyltransferase regulatory gene amplified genomic DNA only from S. oralis strains, but not from any of the other 125 strains tested. Our findings may be useful for the future development of efficient diagnostic tools for the rapid identification and differentiation of S. oralis from other oral streptococci strains.
虽然口腔链球菌的发病机制可能与其他草绿色链球菌不同，但口腔链球菌与这些链球菌具有高度的 DNA 序列相似性。因此，长期以来，口腔链球菌与其近亲的区分一直被认为很困难。本研究旨在寻找特定的基因，以便在体外识别口腔链球菌，而不是其他口腔共生菌。通过抑制性消减杂交获得的 490 个口腔链球菌克隆被用于 Southern 杂交，阳性克隆被测序。在 5 个口腔链球菌特异性克隆中，根据葡糖基转移酶调控基因设计的新引物仅扩增来自口腔链球菌菌株的基因组 DNA，但不扩增测试的其他 125 个菌株。我们的发现可能有助于未来开发有效的诊断工具，用于快速识别和区分口腔链球菌与其他口腔链球菌菌株。
