通过表达过氧化氢酶基因，使长双歧杆菌 105-A 获得对氧化应激的耐受性。
Acquired tolerance to oxidative stress in Bifidobacterium longum 105-A via expression of a catalase gene.
机构信息
The United Graduate School of Agricultural Science, Gifu University, Yanagido, Gifu, Japan.
出版信息
Appl Environ Microbiol. 2012 Apr;78(8):2988-90. doi: 10.1128/AEM.07093-11. Epub 2012 Feb 3.
Abstract
For improvement of tolerance to oxidative stress in Bifidobacterium longum 105-A, we introduced the Bacillus subtilis catalase gene (katE) into it. The transformant showed catalase activity (39 U/mg crude protein) in the intracellular fraction, which increased survival by ∼100-fold after a 1-h exposure to 4.4 mM H(2)O(2), decreased de novo H(2)O(2) accumulation, and increased survival in aerated cultures by 10(5)-fold at 24 h. The protection level was better than that conferred by exogenously added catalase.
摘要
为提高长双歧杆菌 105-A 对氧化应激的耐受性，我们将枯草芽孢杆菌过氧化氢酶基因（katE）导入其中。该转化体在细胞内部分具有过氧化氢酶活性（39 U/mg 粗蛋白），在 1 h 暴露于 4.4 mM H2O2 后，存活率提高了约 100 倍，新生成的 H2O2 积累减少，在 24 h 时通气培养中的存活率提高了 105 倍。保护水平优于外源添加的过氧化氢酶。
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2
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7
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8
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9
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