Department of Microbiology and Parasitology, Faculty of Medical Science, Naresuan University, Phitsanulok 65000, Thailand.
Departamento de Agroquimica y Conservacion de Suelos, Instituto de Recursos Naturales y Agrobiologia de Sevilla, Consejo Superior de Investigaciones Cientificas, Seville 41012, Spain.
Int J Syst Evol Microbiol. 2012 Dec;62(Pt 12):3007-3012. doi: 10.1099/ijs.0.035220-0. Epub 2012 Feb 3.
A novel actinomycete, designated strain PT708(T), was isolated from cave soil collected in Pha Tup Cave Forest Park, Nan province, Thailand. It produced compounds with antimicrobial and anticancer activities. Its chemotaxonomic properties were consistent with those of members of the genus Nonomuraea. The major menaquinone was MK-9(H(4)), with minor amounts of MK-9(H(6)), MK-9(H(2)), MK-10(H(2)) and MK-8(H(4)). The polar lipid profile contained phosphatidylmonomethylethanolamine, diphosphatidylglycerol, hydroxy-phosphatidylmonomethylethanolamine, hydroxy-phosphatidylethanolamine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol mannoside and phosphatidylinositol. The major fatty acids were iso-C(16:0), 10-methyl C(17:0), C(16:0) and C(17:1)ω6c. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain PT708(T) belonged to the genus Nonomuraea and was most closely related to Nonomuraea rhizophila YIM 67092(T) (98.50% sequence similarity) and Nonomuraea rosea GW 12687(T) (98.30%). The genomic DNA G+C content of strain PT708(T) was 73.3 mol%. Unlike the recognized members of the genus Nonomuraea, the novel strain formed single spores at the tips of aerial hyphae. Based on the phenotypic, phylogenetic and genotypic evidence, strain PT708(T) represents a novel species of the genus Nonomuraea, for which the name Nonomuraea monospora sp. nov. is proposed. The type strain is PT708(T) ( = TISTR 1910(T) = JCM 16114(T)).
一株新的放线菌，命名为 PT708(T) 菌株，从泰国喃府 Pha Tup Cave 森林公园采集的洞穴土壤中分离得到。它产生具有抗菌和抗癌活性的化合物。其化学分类特性与野野村菌属的成员一致。主要的类异戊二烯醌为 MK-9(H(4))，少量的 MK-9(H(6))、MK-9(H(2))、MK-10(H(2))和 MK-8(H(4))。极性脂谱含有磷脂单甲基乙醇胺、双磷脂酰甘油、羟基磷脂单甲基乙醇胺、羟基磷脂乙醇胺、磷脂乙醇胺、磷脂酰甘油、磷脂酰肌醇甘露糖苷和磷脂酰肌醇。主要脂肪酸为 iso-C(16:0)、10-甲基 C(17:0)、C(16:0)和 C(17:1)ω6c。基于 16S rRNA 基因序列的系统发育分析表明，PT708(T) 菌株属于野野村菌属，与野野村菌 Nonomuraea rhizophila YIM 67092(T)（98.50%序列相似性）和野野村菌 Nonomuraea rosea GW 12687(T)（98.30%序列相似性）最为密切相关。菌株 PT708(T) 的基因组 DNA G+C 含量为 73.3mol%。与公认的野野村菌属成员不同，新菌株在气生菌丝的顶端形成单个孢子。基于表型、系统发育和基因型证据，PT708(T) 菌株代表野野村菌属的一个新种，命名为野野村单孢子菌 Nonomuraea monospora sp. nov.。模式菌株为 PT708(T)（=TISTR 1910(T)=JCM 16114(T)）。
