Department of Therapy and Rehabilitation, Health Services Vocational College, Kirsehir Ahi Evran University, 44200, Kirsehir, Turkey.
Department of Biology, Faculty of Science and Arts, Ondokuz Mayis University, 55139, Samsun, Turkey.
Arch Microbiol. 2020 Aug;202(6):1535-1543. doi: 10.1007/s00203-020-01866-3. Epub 2020 Mar 31.
A Gram-stain-positive, aerobic, spore-forming actinobacterial strain, designated 160415, was isolated from a surface soil sample, which was formed on basaltic parent material, collected from Samsun, Turkey. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain 160415 clustered closely with species of the genus Nonomuraea, and showed the highest sequence similarity to Nonomuraea zeae NEAU-ND5, Nonomuraea candida HMC10 and Nonomuraea turkmeniaca DSM 43926 with 99.1%, 98.9% and 98.7%, respectively. Chemotaxonomic properties including major menaquinones, diaminopimelic acid, sugar and phospholipid profiles also confirmed the affiliation of the strain to the genus Nonomuraea. The DNA G+C content of strain 160415 was 69.6 mol%. DNA-DNA hybridization and average nucleotide identity values between the strain and closely related type strains were less than the recommended cut-off values. On the basis of phylogenetic relationships, genotypic and phenotypic characterizations, strain 160415 represents a novel species of the genus Nonomuraea, for which the name Nonomuraea basaltis sp. nov. is proposed. The type strain is 160415 (= KCTC 39875 = DSM 104309).
一株革兰氏阳性、需氧、产芽孢放线菌菌株，命名为 160415 号，从土耳其萨姆松采集的玄武岩母质上形成的表层土壤样本中分离得到。基于 16S rRNA 基因序列的系统发育分析表明，菌株 160415 与游动放线菌属的种密切聚类，与玉米游动放线菌 NEAU-ND5、近平滑游动放线菌 HMC10 和土库曼游动放线菌 DSM 43926 的序列相似性最高，分别为 99.1%、98.9%和 98.7%。化学分类特征，包括主要的类异戊二烯醌、二氨基庚二酸、糖和磷脂谱也证实了该菌株与游动放线菌属的归属。菌株 160415 的 DNA G+C 含量为 69.6 mol%。菌株与密切相关的模式株之间的 DNA-DNA 杂交和平均核苷酸同一性值均低于推荐的截止值。基于系统发育关系、基因型和表型特征，菌株 160415 代表游动放线菌属的一个新种，命名为玄武岩游动放线菌（Nonomuraea basaltis）sp. nov.。模式菌株为 160415（=KCTC 39875=DSM 104309）。
