Department of Biology, Faculty of Art and Science, Ondokuz Mayis University, 55139 Kurupelit-Samsun, Turkey.
German Collection of Microorganisms and Cell Cultures GmbH, 38124 Braunschweig, Germany.
Int J Syst Evol Microbiol. 2013 Jan;63(Pt 1):212-218. doi: 10.1099/ijs.0.039362-0. Epub 2012 Mar 2.
A novel actinomycete, strain A4036(T), was isolated from a soil sample collected from the Jabi district in Abuja, Nigeria. The taxonomic position of strain A4036(T) was established using a combination of genotypic and phenotypic analyses. The organism formed extensively branched substrate and aerial hyphae that generated spiral chains of spores with warty surfaces. The cell wall contained meso-diaminopimelic acid and the cell-wall sugars were glucose, madurose, mannose and ribose. The predominant menaquinone was MK-9(H(4)). The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylmethylethanolamine, phosphatidylinositol mannoside, hydroxy-phosphatidylethanolamine, hydroxy-phosphatidylmethylethanolamine, two unidentified phospholipids and four unknown glucosamine-containing phospholipids. The major cellular fatty acids were iso-C(16 : 0) 2-OH, iso-C(16 : 0) and 10-methyl C(17 : 0). On the basis of 16S rRNA gene sequence similarity studies, strain A4036(T) grouped in the genus Nonomuraea, being most closely related to Nonomuraea angiospora IFO 13155(T) (99.05 %), Nonomuraea candida HMC10(T) (98.78 %), Nonomuraea kuesteri GW 14-1925(T) (98.49 %), Nonomuraea endophytica YIM 65601(T) (98.42 %), Nonomuraea maheshkhaliensis 16-5-14(T) (98.40 %), Nonomuraea turkmeniaca DSM 43926(T) (98.38 %), Nonomuraea helvata IFO 14681(T) (98.29 %), Nonomuraea rubra DSM 43768(T) (98.10 %) and Nonomuraea salmonea DSM 43678(T) (98.06 %). Levels of 16S rRNA gene sequence similarity to the type strains of other species of the genus Nonomuraea were <98 %. Despite the high 16S rRNA gene sequence similarities, DNA-DNA relatedness values and phenotypic data demonstrated that strain A4036(T) was clearly distinguished from all closely related species of the genus Nonomuraea. Thus, this isolate is considered to represent a novel species of the genus Nonomuraea, for which the name Nonomuraea jabiensis sp. nov. is proposed. The type strain is A4036(T) (= DSM 45507(T) = KCTC 19870(T)).
一株新的放线菌 A4036(T) 是从尼日利亚阿布贾 Jabi 区的土壤样本中分离得到的。通过基因型和表型分析的结合，确定了菌株 A4036(T) 的分类地位。该菌形成广泛分枝的基质和气生菌丝，产生具有疣状表面的螺旋链孢子。细胞壁含有 meso-二氨基庚二酸，细胞壁糖为葡萄糖、甘露糖、甘露糖和核糖。主要的menaquinone 是 MK-9(H(4))。极性脂为双磷脂酰甘油、磷脂酰乙醇胺、磷脂酰肌醇、磷脂酰甲乙醇胺、磷脂酰肌醇甘露糖苷、羟基磷脂酰乙醇胺、羟基磷脂酰甲乙醇胺、两种未鉴定的磷脂和四种未知的含葡萄糖胺磷脂。主要的细胞脂肪酸为 iso-C(16 : 0) 2-OH、iso-C(16 : 0) 和 10-甲基 C(17 : 0)。根据 16S rRNA 基因序列相似性研究，菌株 A4036(T) 在诺莫拉氏菌属中分组，与诺莫拉氏菌属 angiospora IFO 13155(T)(99.05%)、诺莫拉氏菌属 candida HMC10(T)(98.78%)、诺莫拉氏菌属 kuesteri GW 14-1925(T)(98.49%)、诺莫拉氏菌属 endophytica YIM 65601(T)(98.42%)、诺莫拉氏菌属 maheshkhaliensis 16-5-14(T)(98.40%)、诺莫拉氏菌属 turkmeniaca DSM 43926(T)(98.38%)、诺莫拉氏菌属 helvata IFO 14681(T)(98.29%)、诺莫拉氏菌属 rubra DSM 43768(T)(98.10%)和诺莫拉氏菌属 salmonica DSM 43678(T)(98.06%)最为相似。与该属其他种的模式菌株的 16S rRNA 基因序列相似性水平<98%。尽管 16S rRNA 基因序列高度相似，但 DNA-DNA 同源性值和表型数据表明，菌株 A4036(T)与诺莫拉氏菌属的所有密切相关种明显不同。因此，该分离株被认为代表了一个新的诺莫拉氏菌属种，命名为 Nonomuraea jabiensis sp. nov.。模式菌株为 A4036(T)(=DSM 45507(T)=KCTC 19870(T))。
