Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia, Serdang, Selangor, DE, Malaysia.
Mol Biol Rep. 2012 Jun;39(6):6505-11. doi: 10.1007/s11033-012-1478-2. Epub 2012 Feb 4.
A sum of 48 accessions of physic nut, Jatropha curcas L. were analyzed to determine the genetic diversity and association between geographical origin using RAPD-PCR markers. Eight primers generated a total of 92 fragments with an average of 11.5 amplicons per primer. Polymorphism percentages of J. curcas accessions for Selangor, Kelantan, and Terengganu states were 80.4, 50.0, and 58.7%, respectively, with an average of 63.04%. Jaccard's genetic similarity co-efficient indicated the high level of genetic variation among the accessions which ranged between 0.06 and 0.81. According to UPGMA dendrogram, 48 J. curcas accessions were grouped into four major clusters at coefficient level 0.3 and accessions from same and near states or regions were found to be grouped together according to their geographical origin. Coefficient of genetic differentiation (G(st)) value of J. curcas revealed that it is an outcrossing species.
对 48 份麻疯树（Jatropha curcas L.）材料进行了分析，以 RAPD-PCR 标记确定其遗传多样性和地理起源之间的关系。8 个引物共产生了 92 个片段，每个引物的平均扩增片段数为 11.5 个。雪兰莪州、吉兰丹州和登嘉楼州的麻疯树遗传多样性百分比分别为 80.4%、50.0%和 58.7%，平均为 63.04%。Jaccard 的遗传相似系数表明，这些材料之间存在较高的遗传变异，范围在 0.06 到 0.81 之间。根据 UPGMA 聚类图，48 份麻疯树材料在系数水平 0.3 处分为 4 个主要聚类，来自同一州或邻近州/地区的材料根据其地理起源分组。麻疯树的遗传分化系数（G(st)）值表明其为异交种。
