Food Crops Laboratory, Institute of Tropical Agriculture, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
Biomed Res Int. 2013;2013:604598. doi: 10.1155/2013/604598. Epub 2013 May 21.
The knowledge of genetic diversity of tree crop is very important for breeding and improvement program for the purpose of improving the yield and quality of its produce. Genetic diversity study and analysis of genetic relationship among 20 Moringa oleifera were carried out with the aid of twelve primers from, random amplified polymorphic DNA marker. The seeds of twenty M. oleifera genotypes from various origins were collected and germinated and raised in nursery before transplanting to the field at University Agricultural Park (TPU). Genetic diversity parameter, such as Shannon's information index and expected heterozygosity, revealed the presence of high genetic divergence with value of 1.80 and 0.13 for Malaysian population and 0.30 and 0.19 for the international population, respectively. Mean of Nei's gene diversity index for the two populations was estimated to be 0.20. In addition, a dendrogram constructed, using UPGMA cluster analysis based on Nei's genetic distance, grouped the twenty M. oleifera into five distinct clusters. The study revealed a great extent of variation which is essential for successful breeding and improvement program. From this study, M. oleifera genotypes of wide genetic origin, such as T-01, T-06, M-01, and M-02, are recommended to be used as parent in future breeding program.
对树木作物的遗传多样性的了解对于培育和改良计划非常重要，目的是提高其产量和质量。本研究利用随机扩增多态性 DNA 标记的 12 个引物，对 20 个辣木的遗传多样性进行了研究和分析。从不同来源收集了 20 个辣木基因型的种子，在苗圃中发芽和培育，然后移植到大学农业园区（TPU）的田间。遗传多样性参数，如香农信息指数和期望杂合度，表明存在高遗传分歧，马来西亚种群的值分别为 1.80 和 0.13，国际种群的值分别为 0.30 和 0.19。两个种群的 Nei 基因多样性指数平均值估计为 0.20。此外，根据 Nei 遗传距离构建的 UPGMA 聚类分析构建的系统发育树将 20 个辣木分为五个不同的聚类。该研究揭示了广泛的变异，这对于成功的培育和改良计划至关重要。本研究建议使用 T-01、T-06、M-01 和 M-02 等具有广泛遗传起源的辣木基因型作为未来育种计划的亲本。
