利用光学相干断层扫描技术对 NPC 细胞系进行定量鉴别。

Quantitative discrimination of NPC cell lines using optical coherence tomography.

机构信息

School for Information and Optoelectronic Science and Engineering, South China Normal University, Guangzhou 510006, China.

出版信息

J Biophotonics. 2012 Jul;5(7):544-9. doi: 10.1002/jbio.201100121. Epub 2012 Feb 6.

DOI:10.1002/jbio.201100121

PMID:22308071

Abstract

We tried to explore the intrinsic differences in the optical properties of the four representative NPC cell lines on the models of radiobiology and metastasis by OCT. The scattering coefficients and anisotropies were extracted by fitting the average a-scan attenuation curves based on the multiple scatter effect. The values of scattering coefficients and anisotropy factors were 5.21 ± 0.11, 5.30 ± 0.09, 5.92 ± 0.21, 6.97 ± 0.22, and 0.892 ± 0.009, 0.886 ± 0.006, 0.884 ± 0.009, 0.86 ± 0.01 for CNE1, CNE2, 5-8F and 6-10B pellets (p < 0.05, P = 0.07 for CNE1 and CNE2), respectively. The results showed that the radiobiology and metastasis cell's model could be distinguished obviously; which implied that the corresponding types of NPC tissue might be potentially differentiated by OCT.

摘要

我们试图通过 OCT 探索四种具有代表性的 NPC 细胞系在放射生物学和转移模型上的固有光学性质差异。基于多次散射效应，通过拟合平均 a 扫描衰减曲线来提取散射系数和各向异性。散射系数和各向异性因子的值分别为 5.21±0.11、5.30±0.09、5.92±0.21、6.97±0.22 和 0.892±0.009、0.886±0.006、0.884±0.009、0.86±0.01，用于 CNE1、CNE2、5-8F 和 6-10B 细胞球（p<0.05，CNE1 和 CNE2 为 P=0.07）。结果表明，放射生物学和转移细胞模型可以明显区分；这意味着 OCT 可能潜在地区分 NPC 组织的相应类型。