Li J H, Chen J, Mu X J, Shao Q L, Zhou Y Q, Yan L J
Appl Opt. 2017 Oct 20;56(30):8335-8339. doi: 10.1364/AO.56.008335.
The purpose is to demonstrate the optical charactering concerning nasopharyngeal tissue of pig by fresh sections and frozen correlating sections with optical coherence tomography (OCT). After being imaged on a fresh specimen, samples are then stored in low temperature refrigerators (-80°C) for one year for the second OCT measurement. The OCT structure of the epithelium, lamina propria, and the basement membrane are still resolvable; the median scattering coefficients and anisotropy factors fitting from OCT images based on the multiple scattering effects for epithelium are 27.6 mm [interquartile range (IQR) 23.6 to 29.3 mm] versus 22.5 mm (IQR 20.5 to 24.4 mm), 0.86 (IQR 0.81 to 0.9) versus 0.88 (IQR 0.87 to 0.9) for fresh and frozen tissue, respectively; and 10.2 mm (IQR 8.1 to 13.6 mm) versus 9.6 mm (IQR 8.1 to 13.8 mm), 0.96 (IQR 0.93 to 0.98) versus 0.92 (IQR 0.9 to 0.98) for lamina propria, respectively. The results show that the frozen storage method can be used for OCT research.
目的是通过新鲜切片和光学相干断层扫描（OCT）的冷冻相关切片来展示猪鼻咽组织的光学特征。在新鲜标本上成像后，样本随后储存在低温冰箱（-80°C）中一年以进行第二次OCT测量。上皮、固有层和基底膜的OCT结构仍然可分辨；基于多次散射效应从OCT图像拟合得到的上皮的中位散射系数和各向异性因子，新鲜组织分别为27.6 mm[四分位间距（IQR）23.6至29.3 mm]和22.5 mm（IQR 20.5至24.4 mm），0.86（IQR 0.81至0.9）和0.88（IQR 0.87至0.9）；固有层分别为10.2 mm（IQR 8.1至13.6 mm）和9.6 mm（IQR 8.1至13.8 mm），0.96（IQR 0.93至0.98）和0.92（IQR 0.9至0.98）。结果表明，冷冻保存方法可用于OCT研究。
