Institute for Animal Health, Pirbright Laboratory, Ash Road, Pirbright, Surrey, GU24 0NF, United Kingdom.
J Med Entomol. 2012 Jan;49(1):112-21. doi: 10.1603/me11145.
The host kairomones carbon dioxide (CO2) and 1-octen-3-ol elicit a host seeking response in a wide range of haematophagous Diptera. This study investigates the response of Culicoides biting midges (Diptera: Ceratopogonidae) to these cues using field-based experiments at two sites in the United Kingdom with very different species complements. Traps used for surveillance (miniature CDC model 512) and control (Mosquito Magnet Pro) were modified to release ratios of (R)- and (S)-1-octen-3-ol enantiomers in combination with CO2 and, in the case of the latter trap type, a thermal cue. Abundance and species diversity were then compared between these treatments and against collections made using a trap with a CO2 lure only, in a Latin square design. In both habitats, results demonstrated that semiochemical lures containing a high proportion of the (R)-enantiomer consistently attracted a greater abundance of host-seeking Culicoides females than any other treatment. Culicoides collected using an optimal stimulus of 500 ml/min CO2 combined with 4.1 mg/h (R)-1-octen-3-ol were then compared with those collected on sheep through the use of a drop trap. While preliminary in nature, this trial indicated Culicoides species complements are similar between collections made using the drop trap in comparison to the semiochemical-baited CDC trap, and that there are advantages in using (R)-1-octen-3-ol.
主机信息素二氧化碳（CO2）和 1-辛烯-3-醇在广泛的吸血双翅目昆虫中引发宿主搜索反应。本研究使用英国两个具有非常不同物种组成的地点的现场实验来研究致倦库蚊（双翅目：蠓科）对这些线索的反应。用于监测的诱捕器（微型 CDC 模型 512）和控制（蚊子磁铁 Pro）进行了修改，以释放（R）-和（S）-1-辛烯-3-醇对映体与 CO2 的比例，在后一种诱捕器类型的情况下，还释放了热线索。然后，在拉丁方设计中，将这些处理与仅使用 CO2 诱饵的诱捕器的收集进行了比较，比较了丰度和物种多样性。在这两种生境中，结果表明，含有高比例（R）-对映体的半化学引诱剂诱捕到的宿主搜索致倦库蚊雌蚊数量始终多于任何其他处理。然后将使用 500 ml/min CO2 与 4.1 mg/h（R）-1-辛烯-3-醇组合的最佳刺激收集到的致倦库蚊与使用滴剂陷阱收集到的绵羊上的致倦库蚊进行了比较。虽然这是初步的试验，但该试验表明，与使用半化学引诱剂诱捕的 CDC 诱捕器相比，使用滴剂陷阱收集的致倦库蚊物种组成相似，并且使用（R）-1-辛烯-3-醇具有优势。
