García-Roca P, Vargas Y A, Fuentes Y, Hernández A M, Ortiz L, Valverde S, Velásquez-Jones L, Romero-Navarro B, Ramón-Garcia G, Medeiros M
Laboratorio de Investigación en Nefrología y Metabolismo Mineral, Hospital Infantil de México Federico Gómez, México D.F., Mexico.
Pediatr Transplant. 2012 May;16(3):274-9. doi: 10.1111/j.1399-3046.2012.01645.x. Epub 2012 Feb 7.
The aim of the study was to evaluate whether or not serum levels of soluble interleukin 2 receptor (sIL-2R) predict acute rejection in pediatric recipients. We studied 51 pediatric renal transplant recipients divided into three groups: Group 1) Biopsy-proven cellular acute rejection (n = 19), Group 2) Graft dysfunction with histological diagnosis other than acute rejection (n = 8) and Group 3) Patients with stable graft function, no biopsy (n = 24). Serum samples for sIL-2R measurement by sandwich ELISA were obtained at the time of renal transplant and at the time of renal biopsy due to graft dysfunction (Groups 1 and 2) or at six months post-transplant in the case of Group 3. The mean ± s.e. serum values of sIL-2R were higher in patients during acute rejection (6539 ± 1802 pg/mL) compared to patients with other causes of graft dysfunction (2217 ± 256 pg/mL) or stable graft function at six months (2183 ± 283 pg/mL) (Kruskal-Wallis p = 0.004). When the sIL2-R levels at the time of transplant were compared to those at the time of biopsy (Groups 1 and 2) or at six months post-transplant in Group 3, there was no significant difference between baseline and biopsy in the acute rejection group (paired t-test = 0.07), whereas there was a significant reduction in Groups 2 and 3.
本研究的目的是评估血清可溶性白细胞介素2受体（sIL-2R）水平能否预测小儿肾移植受者的急性排斥反应。我们研究了51名小儿肾移植受者，将其分为三组：第1组）经活检证实的细胞性急性排斥反应（n = 19）；第2组）移植肾功能障碍，组织学诊断不是急性排斥反应（n = 8）；第3组）移植肾功能稳定，未进行活检（n = 24）。在肾移植时以及因移植肾功能障碍（第1组和第2组）进行肾活检时或第3组移植后6个月时采集血清样本，采用夹心酶联免疫吸附测定法（ELISA）检测sIL-2R。与因其他原因导致移植肾功能障碍的患者（2217±256 pg/mL）或移植后6个月移植肾功能稳定的患者（2183±283 pg/mL）相比，急性排斥反应患者的sIL-2R平均±标准误血清值更高（6539±1802 pg/mL）（Kruskal-Wallis检验，p = 0.004）。将移植时的sIL2-R水平与活检时（第1组和第2组）或第3组移植后6个月时的水平进行比较，急性排斥反应组基线水平与活检时水平之间无显著差异（配对t检验= 0.07），而第2组和第3组则有显著降低。
