Department of Microbiology, Yamagata Prefectural Institute of Public Health, Yamagata, Japan.
Microbiol Immunol. 2012 Feb;56(2):139-43. doi: 10.1111/j.1348-0421.2012.00411.x.
To clarify the epidemiology of enterovirus 68 (EV68), which is one of the most rarely identified enteroviruses, virus isolation and molecular screening using RT-PCR was performed on 6307 respiratory specimens collected at pediatric clinics in Yamagata, Japan between 2005 and 2010. In the years 2005-2009, 10, 1, 2, 0, and 2 (40) EV68-positive cases, respectively, were identified by RT-PCR. In 2010, 40 cases were identified altogether: 2 by isolation only, 26 by RT-PCR only, and 12 by both isolation and RT-PCR. Phylogenetic analysis indicated that plural genetically distinct clusters co-circulated. These results suggest that that difficulty in EV68 isolation leads to an underestimation of the prevalence of EV68 infections.
为了阐明肠道病毒 68 型（EV68）的流行病学情况，该病毒是最罕见的肠道病毒之一，我们对 2005 年至 2010 年间日本山形县儿科诊所采集的 6307 份呼吸道标本进行了病毒分离和 RT-PCR 分子筛查。在 2005-2009 年期间，分别通过 RT-PCR 鉴定出 10、1、2、0 和 2 例（共 40 例）EV68 阳性病例。在 2010 年，共鉴定出 40 例：2 例仅通过分离鉴定，26 例仅通过 RT-PCR 鉴定，12 例通过分离和 RT-PCR 鉴定。系统进化分析表明，多个遗传上不同的簇共同传播。这些结果表明，EV68 分离的困难导致了对 EV68 感染的流行率的低估。
