Ishikawa Shintaro, Suga Hiroki, Fukushima Masaya, Yoshida Atsuhiro, Yoshida Yuri, Sunagawa Masataka, Hisamitsu Tadashi
Department of Physiology, School of Medicine, Showa University, Tokyo, Japan.
J Acupunct Meridian Stud. 2012 Feb;5(1):21-8. doi: 10.1016/j.jams.2011.11.003. Epub 2011 Dec 17.
We have reported that electrical acupuncture stimulation (ACU) increases blood fluidity by decreasing platelet aggregation. In this study, we investigated the mechanism causing the increase of blood fluidity. The effects of ACU on blood fluidity and platelet adhesion were examined using a Micro Channel Array Flow Analyzer (MC-FAN) and a laser scattering platelet aggregometer (PA-20). Male Wistar rats (7-8 weeks old) were used in the study. ACU (1 or 100 Hz, 3-5 V), which causes slight muscle twitching, was applied to the ZuSanli (ST-36) acupoint for 15 or 60 minutes once/day. Blood samples were collected from the inferior vena cava. ACU applied to ST-36 revealed significant increases in blood fluidity, while platelet adhesion activity decreased, regardless of the difference of stimulus time. The acupuncture had an immediate effect. Even if naloxone was administered during acupuncture stimulus, the blood flow time shortened in a similar way, as in the only acupuncture stimulus group. In addition, the effect of acupuncture on blood fluidity was inhibited by a β-antagonist. The results indicate that ACU affects blood fluidity depending on the acupoints, and that the effect of ACU might involve an endogenous adrenergic mechanism.
我们曾报道，电针刺激（ACU）通过降低血小板聚集来增加血液流动性。在本研究中，我们探究了导致血液流动性增加的机制。使用微通道阵列流动分析仪（MC - FAN）和激光散射血小板聚集仪（PA - 20）检测了电针刺激对血液流动性和血小板黏附的影响。研究采用雄性Wistar大鼠（7 - 8周龄）。将引起轻微肌肉抽搐的电针刺激（1或100 Hz，3 - 5 V）施加于足三里（ST - 36）穴位，每天一次，持续15或60分钟。从下腔静脉采集血样。无论刺激时间长短，施加于ST - 36的电针刺激均使血液流动性显著增加，同时血小板黏附活性降低。针刺具有即时效应。即使在针刺刺激期间给予纳洛酮，血流时间仍以与仅针刺刺激组相似的方式缩短。此外，针刺对血液流动性的影响被β受体拮抗剂抑制。结果表明，电针刺激根据穴位不同影响血液流动性，且电针刺激的作用可能涉及内源性肾上腺素能机制。
