噬菌体展示用噬菌粒载体：性质、特征与构建。

Phagemid vectors for phage display: properties, characteristics and construction.

机构信息

Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, China.

出版信息

J Mol Biol. 2012 Mar 30;417(3):129-43. doi: 10.1016/j.jmb.2012.01.038. Epub 2012 Jan 30.

DOI:10.1016/j.jmb.2012.01.038

Abstract

Phagemids are filamentous-phage-derived vectors containing the replication origin of a plasmid. Phagemids usually encode no or only one kind of coat proteins. Other structural and functional proteins necessary to accomplish the life cycle of phagemid are provided by the helper phage. In addition, other elements such as molecular tags and selective markers are introduced into the phagemids to facilitate the subsequent operations, such as gene manipulation and protein purification. This review summarizes the elements of the phagemids and their corresponding functions. Finally, the possible trends and future direction to improve the characteristics of the phagemids are highlighted.

摘要

噬菌粒是丝状噬菌体衍生的载体，其中包含质粒的复制原点。噬菌粒通常不编码或仅编码一种衣壳蛋白。完成噬菌粒生命周期所需的其他结构和功能蛋白由辅助噬菌体提供。此外，还引入了分子标记和选择性标记等其他元件到噬菌粒中，以方便后续操作，如基因操作和蛋白质纯化。本文综述了噬菌粒的元件及其相应功能。最后，强调了改善噬菌粒特性的可能趋势和未来方向。