Mead D A, Kemper B
Biotechnology. 1988;10:85-102. doi: 10.1016/b978-0-409-90042-2.50010-6.
A variety of ssDNA phagemid cloning vectors have been constructed that combine the advantages of the filamentous coliphages with a number of plasmid and other bacteriophage-encoded functions. The practical and biological advantages of a chimeric phage-plasmid vector are considerable, and the trend toward converting existing plasmids into ssDNA phagemids and consolidating any number of useful features into one or a few vectors will undoubtedly accelerate. A new helper phage specifically designed for the isolation of large amounts of single-stranded plasmid DNA simplifies the use of these cloning vehicles. Additional refinements in phagemid-helper phage systems should extend the potential of these vectors even further.
已经构建了多种单链DNA噬菌粒克隆载体，这些载体结合了丝状大肠杆菌噬菌体的优点以及许多质粒和其他噬菌体编码的功能。嵌合噬菌体-质粒载体的实际和生物学优势相当大，将现有质粒转化为单链DNA噬菌粒并将大量有用特征整合到一个或几个载体中的趋势无疑将加速。一种专门设计用于分离大量单链质粒DNA的新型辅助噬菌体简化了这些克隆载体的使用。噬菌粒-辅助噬菌体系统的进一步改进应能进一步扩展这些载体的潜力。
