Allergy Unit, Pneumology and Respiratory Allergy Department, Thorax Institute, Hospital Clinic, Universitat de Barcelona, Barcelona, Spain.
J Investig Allergol Clin Immunol. 2011;21(7):522-6.
Lipid transfer proteins (LTP) are responsible for systemic manifestations in food allergy. Their relationship with pollinosis is not clear. In our area, many patients allergic to multiple LTP-containing foods present pollinosis due to Cupressus arizonica.
We selected 6 patients with cypress pollinosis and food allergy to peach. Skin prick tests (SPT) were performed for pollens (grass, cypress, wall pellitory, plane tree, and olive tree) and plant foods (hazelnut, kiwifruit, peach peel, maize, wheat, peanut, lettuce, apple, mustard, and melon). In vitro assays included specific immunoglobulin (Ig) E to C arizonica and peach LTP (Pru p 3), enzyme allergosorbent test (EAST) inhibition, immunoblotting, immunoblotting-inhibition, and immunocytochemical techniques for the detection of Pru p 3-like LTP in cypress pollen grains.
SPT were positive for C arizonica, peach, lettuce, mustard, and hazelnut in all patients. Specific IgE to C arizonica and Pru p 3 was positive in all but 1 patient, whose Pru p 3 IgE was negative. Immunoblotting under nonreducing conditions with C arizonica extract and patients' sera showed a band at 14-15 kDa that was inhibited by Pru p 3. Pru p 3 partially inhibited the C arizonica pollen extract in EAST-inhibition. Pru p 3-like LTP was localized in the cytoplasm and walls of C arizonica pollen grains.
A 15-kDa allergen in C arizonica pollen was found in a group of patients presenting peach allergy and respiratory symptoms to cypress. In vitro tests and immunocytochemical techniques indicate that this protein is an LTP.
脂质转移蛋白（LTP）是食物过敏全身表现的原因。它们与花粉症的关系尚不清楚。在我们地区，许多对多种含 LTP 的食物过敏的患者因接触侧柏而出现花粉症。
我们选择了 6 例侧柏花粉过敏和桃过敏的患者。对花粉（草、侧柏、墙藜、悬铃木和橄榄树）和植物性食物（榛子、猕猴桃、桃皮、玉米、小麦、花生、生菜、苹果、芥菜和瓜）进行皮肤点刺试验（SPT）。体外检测包括对侧柏和桃 LTP（Pru p 3）的特异性免疫球蛋白 E（IgE）、酶过敏原吸附试验（EAST）抑制、免疫印迹、免疫印迹抑制和免疫细胞化学技术，以检测侧柏花粉粒中的 Pru p 3 样 LTP。
所有患者的侧柏、桃、生菜、芥菜和榛子 SPT 均为阳性。除 1 例患者外，其余患者均对侧柏和 Pru p 3 的特异性 IgE 呈阳性，该患者的 Pru p 3 IgE 为阴性。在非还原条件下用侧柏提取物和患者血清进行免疫印迹显示 14-15 kDa 处有一条带，该条带可被 Pru p 3 抑制。Pru p 3 部分抑制 EAST 抑制中的侧柏花粉提取物。Pru p 3 样 LTP 定位于侧柏花粉粒的细胞质和壁中。
我们在一组表现出桃过敏和对侧柏呼吸道症状的患者中发现了侧柏花粉中的 15 kDa 过敏原。体外试验和免疫细胞化学技术表明该蛋白为 LTP。
