The Center of Excellence in Biotechnology Research, King Saud University, Riyadh, Saudi Arabia.
J Investig Allergol Clin Immunol. 2011;21(7):551-5.
Asthma is a multifactorial disorder, and both genetic and environmental factors contribute to its development. We investigated the possible association between asthma and 5 single-nucleotide polymorphisms (SNPs) in the interleukin 17 (IL17) gene--rs17880588 (G/A) and rs17878530 (C/T) in IL17A and rs763780 (T/C), rs11465553 (T/C), and rs2397084 (G/A) in IL17F--and compared levels of the proteins IL17A and IL17F in asthma patients with those of controls.
The study group included 100 asthma patients and 102 ethnically matched controls. Genotyping was performed on purified DNA using reverse transcriptase-polymerase chain reaction with specific primers and probes. Levels of IL17A and IL17F were measured in plasma using enzyme-linked immunosorbent assay.
Genotyping showed that AG heterozygotes of rs17880588 in IL17A were significantly more common in the control group than among the asthma patients (P < .05); no significant associations were observed for any of the other SNPs examined. Levels of IL17A and IL17F were both higher in asthma patients (IL17A, 2.242 [0.099] vs 2.752 [0.287] pg/mL; IL17F, 236.01 [38.28] vs 700 [201.078] pg/mL). The difference was statistically significant for IL17F (P = .025, t test). Levels of IL17A and IL17F were positively and significantly correlated in the asthma patients
Of all the SNPs analyzed, only rs17880588 showed a significant association with asthma in the Saudi population we studied. Levels of IL17A and IL17F were significantly upregulated in the asthma patients. The morphology of IL17F appeared to affect expression levels.
哮喘是一种多因素疾病，遗传和环境因素都可能导致其发病。我们研究了白细胞介素 17（IL17）基因中的 5 个单核苷酸多态性（SNP）与哮喘之间的可能关联，这些 SNP 包括 IL17A 中的 rs17880588（G/A）和 rs17878530（C/T），IL17F 中的 rs763780（T/C）、rs11465553（T/C）和 rs2397084（G/A），并比较了哮喘患者和对照组的 IL17A 和 IL17F 蛋白水平。
研究组包括 100 例哮喘患者和 102 例匹配的对照组。采用逆转录聚合酶链反应，用特异性引物和探针对纯化的 DNA 进行基因分型。采用酶联免疫吸附试验检测血浆中 IL17A 和 IL17F 的水平。
基因分型显示，IL17A 中的 rs17880588 的 AG 杂合子在对照组中比哮喘患者中更为常见（P <.05）；而对于其他检查的 SNP 则未观察到显著相关性。哮喘患者的 IL17A 和 IL17F 水平均升高（IL17A，2.242 [0.099] 与 2.752 [0.287] pg/ml；IL17F，236.01 [38.28] 与 700 [201.078] pg/ml）。IL17F 的差异有统计学意义（P =.025，t 检验）。哮喘患者的 IL17A 和 IL17F 水平呈正相关且有统计学意义。
在我们研究的沙特人群中，所有分析的 SNP 中，只有 rs17880588 与哮喘有显著关联。哮喘患者的 IL17A 和 IL17F 水平明显上调。IL17F 的形态似乎影响表达水平。
