Fatahi F, Chaleshtori Ars, Samani K Ghatreh, Mousavi S M, Zandi F, Heydari S, Hashemzadeh Chaleshtori M, Amiri M, Khazraee H
Department of Microbiology and Immunology, Kurdistan University of Medical Sciences, Sanandaj, Iran.
Department of Epidemiology and Biostatistics, School of Health, Shahrekord University of Medical Sciences, Shahrekord, Iran.
Ann Med Health Sci Res. 2016 Jul-Aug;6(4):216-223. doi: 10.4103/amhsr.amhsr_485_14.
The genes encoding , , , and have recently been implicated in the genetic basis of rhinitis and allergy.
The purpose of this study was to assess the association of the single nucleotide polymorphisms (SNPs) of IL9, IL9R, IL17A, and IL17F and potential interaction of these genes with the determination of IgE levels in women with allergic rhinitis (AR) in Shahrekord, Iran.
In a case-control study, SNPs from the , , , and were genotyped in 394 random samples including 195 AR patients and 199 normal controls. Enzyme-linked immunosorbent assay was performed for the determination of serum total IgE levels. The Student's -test was used to compare the differences. The Chi-square test was performed to compare proportions of cases with different clinical features among cases with different genotypes. The genotype and allele frequencies were obtained by direct counting. Hardy-Weinberg equilibrium was tested between cases and controls separately. The relative risk associated with rare alleles was estimated as an odds ratio with 95% confidence interval. ≤ 0.05 was considered statistically significant.
The rs731476 SNP in the IL9R was significantly associated with the AR phenotype in women. No association was found between any of the other SNPs in , , and genes and AR. In the gene-gene interaction analysis, we found that / genotype rs731476 T-/rs2069885 G conferred a higher risk for AR phenotype development. We also did not find a significant association in terms of IgE levels between cases and controls.
Our result suggests that the rs731476 SNP located in the IL9R is associated with an increased susceptibility to AR in females. In a subsequent gene-gene interaction analysis, the rs731476 T-/rs2069885 G-genotype combination (IL9R/IL9) has significantly been associated with the development of the AR phenotype.
编码白细胞介素9（IL9）、白细胞介素9受体（IL9R）、白细胞介素17A（IL17A）和白细胞介素17F（IL17F）的基因最近被认为与鼻炎和过敏的遗传基础有关。
本研究的目的是评估伊朗沙赫雷克德地区变应性鼻炎（AR）女性患者中IL9、IL9R、IL17A和IL17F的单核苷酸多态性（SNP）的相关性以及这些基因之间的潜在相互作用与免疫球蛋白E（IgE）水平测定的关系。
在一项病例对照研究中，对394个随机样本（包括195例AR患者和199例正常对照）进行IL9、IL9R、IL17A和IL17F基因的SNP基因分型。采用酶联免疫吸附测定法测定血清总IgE水平。使用学生t检验比较差异。采用卡方检验比较不同基因型病例中具有不同临床特征的病例比例。通过直接计数获得基因型和等位基因频率。分别在病例组和对照组中检验哈迪-温伯格平衡。将与罕见等位基因相关的相对风险估计为比值比，并给出95%置信区间。P≤0.05被认为具有统计学意义。
IL9R基因中的rs731476 SNP与女性AR表型显著相关。在IL9、IL17A和IL17F基因的其他任何SNP与AR之间均未发现相关性。在基因-基因相互作用分析中，我们发现IL9R/IL9基因型rs731476 T-/rs2069885 G赋予AR表型发展更高的风险。我们在病例组和对照组的IgE水平方面也未发现显著相关性。
我们的结果表明，位于IL9R基因中的rs731476 SNP与女性AR易感性增加有关。在随后的基因-基因相互作用分析中，rs731476 T-/rs2069885 G基因型组合（IL9R/IL9）与AR表型的发展显著相关。
