Department of Life and Nanopharamceutical Science, Kyung Hee University, Seoul 130-701, Republic of Korea.
J Ethnopharmacol. 2012 Mar 27;140(2):391-7. doi: 10.1016/j.jep.2012.01.032. Epub 2012 Jan 31.
Deer antler has been used for centuries as medicine for a wide range of health problems, including various women's diseases. However, there is a safety concern related to its use in hormone-sensitive conditions, such as breast cancer and endometriosis. In the present study, we investigated the effect of deer (Cervus elaphus) antler extract (DAE) on adhesion and migration of human endometriotic cells.
Adhesion, wound-healing, and transwell migration assays were performed in endometriotic cells 11Z and 12Z. Expression of matrix metalloproteinase (MMP)-2, MMP-9, TNF-α, and IL-6 were measured by real-time RT-PCR and Western blot analysis.
DAE (50 and 100μg/ml) decreased the adhesion of 11Z and 12Z cells on peritoneal mesothelial Met5-A cells. Wound-healing and transwell migration assays revealed that DAE (50 and 100μg/ml) inhibited migration in 11Z and 12Z cells. It was further demonstrated that treatment with DAE (50 and 100μg/ml) significantly decreased the levels of MMP-2, MMP-9, TNF-α, and IL-6.
These results indicate that DAE is a potential anti-endometriotic agent to inhibit the adhesion and migration of endometrial cells through the suppression of various related molecules.
鹿角已被使用了数个世纪，作为治疗各种健康问题的药物，包括各种妇科疾病。然而，其在激素敏感条件下的使用存在安全性问题，例如乳腺癌和子宫内膜异位症。在本研究中，我们研究了鹿（Cervus elaphus）鹿角提取物（DAE）对人子宫内膜异位细胞黏附和迁移的影响。
在子宫内膜异位细胞 11Z 和 12Z 中进行黏附、划痕愈合和 Transwell 迁移测定。通过实时 RT-PCR 和 Western blot 分析测量基质金属蛋白酶（MMP）-2、MMP-9、TNF-α 和 IL-6 的表达。
DAE（50 和 100μg/ml）降低了 11Z 和 12Z 细胞在腹膜间皮 Met5-A 细胞上的黏附。划痕愈合和 Transwell 迁移测定表明，DAE（50 和 100μg/ml）抑制了 11Z 和 12Z 细胞的迁移。进一步表明，用 DAE（50 和 100μg/ml）处理显著降低了 MMP-2、MMP-9、TNF-α 和 IL-6 的水平。
这些结果表明，DAE 是一种潜在的抗子宫内膜异位药物，可通过抑制各种相关分子来抑制子宫内膜细胞的黏附和迁移。
