脊髓灰质炎病毒复制酶：一种能够起始脊髓灰质炎病毒RNA复制的可溶性酶。
Poliovirus replicase: a soluble enzyme able to initiate copying of poliovirus RNA.
作者信息
Dasgupta A, Baron M H, Baltimore D
出版信息
Proc Natl Acad Sci U S A. 1979 Jun;76(6):2679-83. doi: 10.1073/pnas.76.6.2679.
Abstract
The soluble phase of the cytoplasm of poliovirus-infected cells contains an enzymatic activity able to copy RNA without an added primer. This replicase activity has been purified 60-fold; it is absent from uninfected cells. Poly(U) polymerase activity copurifies with replicase activity. Although less pure replicase fractions copy a variety of RNAs, purer fractions respond better to poliovirus RNA than to other viral RNAs. Even the less pure fractions make a specific copy of the added template, as shown by hybridization of the product to its template RNA but not to other RNAs. Among homopolymers only poly(A)-oligo(U) was copied by the replicase; other primed homopolymer templates were inactive.
摘要
脊髓灰质炎病毒感染细胞的细胞质可溶性部分含有一种无需添加引物就能复制RNA的酶活性。这种复制酶活性已被纯化了60倍；未感染的细胞中不存在这种活性。聚尿苷酸聚合酶活性与复制酶活性共同纯化。尽管纯度较低的复制酶组分能复制多种RNA，但纯度较高的组分对脊髓灰质炎病毒RNA的反应比对其他病毒RNA的反应更好。即使是纯度较低的组分也能对添加的模板进行特异性复制，这通过产物与模板RNA而非其他RNA的杂交得以证明。在同聚物中，只有聚腺苷酸 - 寡聚尿苷酸能被复制酶复制；其他带引物的同聚物模板则无活性。
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