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脊髓灰质炎病毒RNA依赖性RNA聚合酶在体外能合成脊髓灰质炎病毒RNA、细胞信使核糖核酸（mRNA）以及几种植物病毒RNA的全长拷贝。

Poliovirus RNA-dependent RNA polymerase synthesizes full-length copies of poliovirion RNA, cellular mRNA, and several plant virus RNAs in vitro.

作者信息

Tuschall D M, Hiebert E, Flanegan J B

出版信息

J Virol. 1982 Oct;44(1):209-16. doi: 10.1128/JVI.44.1.209-216.1982.

DOI:10.1128/JVI.44.1.209-216.1982
PMID:6183446
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC256254/
Abstract

The poliovirus RNA-dependent RNA polymerase was active on synthetic homopolymeric RNA templates as well as on every natural RNA tested. The polymerase copied polyadenylate. oligouridylate [oligo(U)], polycytidylate . oligoinosinate, and polyinosinate. oligocytidylate templates to about the same extent. The observed activity on polyuridylate. oligoadenylate was about fourfold less. Full-length copies of both poliovirion RNA and a wide variety of other polyadenylated RNAs were synthesized by the polymerase in the presence of oligo(U). Polymerase elongation rates on poliovirion RNA and a heterologous RNA (squash mosaic virus RNA) were about the same. Changes in the Mg(2+) concentration affected the elongation rates on both RNAs to the same extent. With two non-polyadenylated RNAs (tobacco mosaic virus RNA and brome mosaic virus RNA3), the results were different. The purified polymerase synthesized a subgenomic-sized product RNA on brome mosaic virus RNA3 in the presence of oligo(U). This product RNA appeared to initiate on oligo(U) hybridized to an internal oligoadenylate sequence in brome mosaic virus RNA3. No oligo(U)-primed product was synthesized on tobacco mosaic virus RNA. When partially purified polymerase was used in place of the completely purified enzyme, some oligo(U)-independent activity was observed on the brome mosaic virus and tobacco mosaic virus RNAs. The size of the product RNA from these reactions suggested that at least some of the product RNA was full-sized and covalently linked to the template RNA. Thus, the polymerase was found to copy many different types of RNA and to make full-length copies of the RNAs tested.

摘要

脊髓灰质炎病毒RNA依赖性RNA聚合酶对合成的同聚RNA模板以及所测试的每种天然RNA都有活性。该聚合酶能复制聚腺苷酸、寡聚尿苷酸[oligo(U)]、聚胞苷酸、寡聚肌苷酸以及聚肌苷酸。寡聚胞苷酸模板，复制程度大致相同。在聚尿苷酸。寡聚腺苷酸上观察到的活性约低四倍。在oligo(U)存在的情况下，该聚合酶合成了脊髓灰质炎病毒RNA以及多种其他聚腺苷酸化RNA的全长拷贝。脊髓灰质炎病毒RNA和异源RNA（南瓜花叶病毒RNA）上的聚合酶延伸速率大致相同。Mg(2+)浓度的变化对两种RNA的延伸速率影响程度相同。对于两种非聚腺苷酸化RNA（烟草花叶病毒RNA和雀麦花叶病毒RNA3），结果有所不同。纯化的聚合酶在oligo(U)存在的情况下，在雀麦花叶病毒RNA3上合成了亚基因组大小的产物RNA。该产物RNA似乎起始于与雀麦花叶病毒RNA3内部寡聚腺苷酸序列杂交的oligo(U)。在烟草花叶病毒RNA上未合成oligo(U)引发的产物。当使用部分纯化的聚合酶代替完全纯化的酶时，在雀麦花叶病毒和烟草花叶病毒RNA上观察到了一些不依赖oligo(U)的活性。这些反应产生的产物RNA的大小表明，至少一些产物RNA是全长的并且与模板RNA共价连接。因此，发现该聚合酶能复制许多不同类型的RNA，并能对所测试的RNA进行全长复制。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/15c4/256254/ef9e52fda0af/jvirol00151-0225-a.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/15c4/256254/ef9e52fda0af/jvirol00151-0225-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/15c4/256254/b6ac621000e8/jvirol00151-0222-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/15c4/256254/5fe0e0513a48/jvirol00151-0222-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/15c4/256254/0c9bc3cf07c0/jvirol00151-0223-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/15c4/256254/06f04b5e85f5/jvirol00151-0224-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/15c4/256254/ef9e52fda0af/jvirol00151-0225-a.jpg

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