能够复制聚腺苷酸（poly(A)）的脊髓灰质炎病毒特异性引物依赖性RNA聚合酶。
Poliovirus-specific primer-dependent RNA polymerase able to copy poly(A).
作者信息
Flanegan J B, Baltimore D
出版信息
Proc Natl Acad Sci U S A. 1977 Sep;74(9):3677-80. doi: 10.1073/pnas.74.9.3677.
Abstract
A template-dependent RNA polymerase has been isolated from poliovirus-infected cells by assaying for the ability of the enzyme to copy poly(A) complexed to an oligo(U) primer. The polymerase was solubilized with detergent, and RNA was removed by precipitation with 2 M LiCl. The solubilized polymerase required both poly(A) and oligo(U) for activity and was stimulated by Mg2+ but was inhibited by Mn2+. Poly(A)-oligo(U)-dependent poly(U) polymerase was not found in extracts of HeLa cells until about 2 hr after poliovirus infection, and then there was a linear increase in activity until about 5 hr. Analysis of the polymerase by glycerol gradient centrifugation showed that the majority of the activity sedimented at about 4 S, indicating that it was no longer complexed with high-molecular-weight RNA or cellular membranes. This poly(A)-oligo(U)-dependent polymerase activity could represent an important component of the poliovirus RNA-dependent RNA polymerase.
摘要
通过检测该酶复制与寡聚（U）引物复合的多聚（A）的能力，从脊髓灰质炎病毒感染的细胞中分离出了一种依赖模板的RNA聚合酶。用去污剂使该聚合酶溶解，并用2M LiCl沉淀去除RNA。溶解的聚合酶的活性需要多聚（A）和寡聚（U）两者，并且受到Mg2+的刺激，但受到Mn2+的抑制。直到脊髓灰质炎病毒感染后约2小时，在HeLa细胞提取物中才发现依赖多聚（A）-寡聚（U）的多聚（U）聚合酶，然后活性呈线性增加直至约5小时。通过甘油梯度离心分析该聚合酶表明，大部分活性在约4S处沉降，这表明它不再与高分子量RNA或细胞膜复合。这种依赖多聚（A）-寡聚（U）的聚合酶活性可能代表脊髓灰质炎病毒RNA依赖的RNA聚合酶的一个重要成分。
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