Ando Minoru, Kado Shoichi, Suzuki Tomo, Yamamoto Ryota, Nagata Yuriko, Hata Chie, Uchida Kazumi, Kaneko Kimiyuki
Yakult Central Institute for Microbiological Research, 1796 Yaho, Kunitachi-shi, Tokyo 186-8650, Japan.
J Toxicol Pathol. 2011 Dec;24(4):251-5. doi: 10.1293/tox.24.251. Epub 2012 Jan 7.
A subcutaneous tumor was found in the right abdomen of a 16-week-old male TCRβ and p53 double-knockout mouse. The tumor had indistinct borders with the surrounding tissue. The cut surface after formalin fixation was pale yellowish white, partially dark red and partly white. Histologically, the tumor was composed of three distinct regions. The first region showed pleomorphic cells arranged in sheets. The second region showed spindle cells arranged in interlacing fascicles. The final region contained a mixture of the above mentioned two types of cells. Furthermore, a small amount of collagen fibers, round cells, multinucleated giant cells, and cells with eosinophilic granules were observed between these tumor cells. Immunohistochemical examination and electron microscopy identified that the pleomorphic cells and spindle cells were histiocytes and fibroblasts, respectively, and that the round cells were undifferentiated mesenchymal cells. Based on these findings, the tumor was diagnosed as a malignant fibrous histiocytoma.
在一只16周龄的雄性TCRβ和p53双敲除小鼠的右腹部发现了一个皮下肿瘤。该肿瘤与周围组织边界不清。福尔马林固定后的切面为淡黄色白色，部分暗红色，部分白色。组织学上，肿瘤由三个不同区域组成。第一个区域显示多形性细胞呈片状排列。第二个区域显示梭形细胞呈交织束状排列。最后一个区域包含上述两种类型细胞的混合物。此外，在这些肿瘤细胞之间观察到少量胶原纤维、圆形细胞、多核巨细胞和嗜酸性颗粒细胞。免疫组织化学检查和电子显微镜鉴定多形性细胞和梭形细胞分别为组织细胞和成纤维细胞，圆形细胞为未分化间充质细胞。基于这些发现，该肿瘤被诊断为恶性纤维组织细胞瘤。
