Department of Biochemistry and Biophysics, Stockholm University, Stockholm, Sweden.
Sensors (Basel). 2010;10(4):2626-37. doi: 10.3390/s100402626. Epub 2010 Mar 25.
Fourier transform infrared (FTIR) spectroscopy provides a direct, "on-line" monitor of enzymatic reactions. Measurement of enzymatic activity is based on the fact that the infrared spectra of reactants and products of an enzymatic reaction are usually different. Several examples are given using the enzymes pyruvate kinase, fumarase and alcohol dehydrogenase. The main advantage of the infrared method is that it observes the reaction of interest directly, i.e., no activity assay is required to convert the progress of the reaction into an observable quantity.
傅里叶变换红外（FTIR）光谱法为酶反应提供了一种直接的、“在线”监测手段。酶活性的测量基于这样一个事实，即酶反应的反应物和产物的红外光谱通常是不同的。使用丙酮酸激酶、延胡索酸酶和醇脱氢酶等几种酶作为示例。红外方法的主要优点是它可以直接观察感兴趣的反应，即不需要活性测定将反应的进展转化为可观察的量。
