Hajimiri Hasti, Safiabadi Tali Seyed Hamid, Al-Kassawneh Muna, Sadiq Zubi, Jahanshahi-Anbuhi Sana
Department of Chemical and Materials Engineering, Gina Cody School of Engineering, Concordia University, Montreal, QC H3G 2W1, Canada.
Biosensors (Basel). 2023 Sep 19;13(9):893. doi: 10.3390/bios13090893.
The colorimetric detection of glucose in urine through enzymatic reactions offers a low-cost and non-invasive method to aid in diabetes management. Nonetheless, the vulnerability of enzymes to environmental conditions, particularly elevated temperatures, and their activity loss pose significant challenges for transportation and storage. In this work, we developed a stable and portable tablet sensor as a user-friendly platform for glucose monitoring. This innovative device encapsulates glucose oxidase and horseradish peroxidase enzymes with dextran, transforming them into solid tablets and ensuring enhanced stability and practicality. The enzymatic tablet-based sensor detected glucose in urine samples within 5 min, using 3,3',5,5'-tetramethylbenzidine (TMB) as the indicator. The tablet sensor exhibited responsive performance within the clinically relevant range of 0-6 mM glucose, with a limit of detection of 0.013 mM. Furthermore, the tablets detected glucose in spiked real human urine samples, without pre-processing, with high precision. Additionally, with regard to thermal stability, the enzyme tablets better maintained their activity at an elevated temperature as high as 60 °C compared to the solution-phase enzymes, demonstrating the enhanced stability of the enzymes under harsh conditions. The availability of these stable and portable tablet sensors will greatly ease the transportation and application of glucose sensors, enhancing the accessibility of glucose monitoring, particularly in resource-limited settings.
通过酶促反应对尿液中的葡萄糖进行比色检测，为糖尿病管理提供了一种低成本且非侵入性的方法。然而，酶对环境条件尤其是高温的敏感性以及其活性丧失，给运输和储存带来了重大挑战。在这项工作中，我们开发了一种稳定且便携的片剂传感器，作为用于葡萄糖监测的用户友好平台。这种创新设备将葡萄糖氧化酶和辣根过氧化物酶与葡聚糖封装在一起，将它们转化为固体片剂，并确保了更高的稳定性和实用性。基于酶片剂的传感器使用3,3',5,5'-四甲基联苯胺（TMB）作为指示剂，在5分钟内检测尿液样本中的葡萄糖。该片剂传感器在0-6 mM葡萄糖的临床相关范围内表现出响应性能，检测限为0.013 mM。此外，这些片剂无需预处理就能高精度地检测加标的真实人类尿液样本中的葡萄糖。另外，在热稳定性方面，与溶液相酶相比，酶片剂在高达60°C的高温下能更好地保持其活性，证明了酶在恶劣条件下具有更高的稳定性。这些稳定且便携的片剂传感器的可用性将极大地简化葡萄糖传感器的运输和应用，提高葡萄糖监测的可及性，尤其是在资源有限的环境中。
