Department of Pathology, University of Campinas/UNICAMP, Campinas, Brazil.
Histopathology. 2012 Apr;60(5):816-25. doi: 10.1111/j.1365-2559.2011.04128.x. Epub 2012 Feb 9.
To compare the expression of proteins regulated by hypoxia between adenoid cystic carcinoma (ACC) with and without high-grade transformation (HGT).
In eight ACC-HGT and 18 ACC without HGT, expression of hypoxia-inducible factor-1 (HIF-1α), vascular endothelial growth factor (VEGF), glucose transporter-1 (GLUT-1) and microvascular density (MVD) by CD105 (a hypoxia-inducible protein expressed in angiogenic endothelial cells) was determined. Expression levels of HIF-1α and VEGF as well as CD105-MVD did not differ significantly between: (i) transformed and conventional areas (TA and CA, respectively) of ACC-HGT, (ii) CA and ordinary ACC. HIF-1α was detected in 100% of cases and presented a diffuse expression pattern. No significant association was found between levels of HIF-1α expression and tumour size, metastasis and recurrence. GLUT-1 showed a prostromal expression pattern and was observed exclusively in TA (three of six cases) and in only three of 14 ACC.
Both the absence of significant alterations in levels of expression of HIF-1α, VEGF and CD105 and the patterns of expression of HIF-1α and GLUT-1 suggest that hypoxia may not play a key role in the process of high-grade transformation of ACC. Although HIF-1α expression is a common finding in ACC, it cannot be used as a marker of tumour aggressiveness.
比较伴有和不伴有高级别转化（HGT）的腺样囊性癌（ACC）中受缺氧调节的蛋白表达。
在 8 例 ACC-HGT 和 18 例无 HGT 的 ACC 中，通过 CD105（在血管生成性内皮细胞中表达的缺氧诱导蛋白）测定缺氧诱导因子-1（HIF-1α）、血管内皮生长因子（VEGF）、葡萄糖转运蛋白-1（GLUT-1）和微血管密度（MVD）的表达。HIF-1α 和 VEGF 的表达水平以及 CD105-MVD 在 ACC-HGT 的转化区（TA）和常规区（CA）之间以及 CA 和普通 ACC 之间没有显著差异。HIF-1α 在 100%的病例中均有表达，并呈弥漫性表达模式。HIF-1α 表达水平与肿瘤大小、转移和复发之间没有显著关联。GLUT-1 呈间质表达模式，仅在 TA（6 例中的 3 例）和 14 例 ACC 中的 3 例中观察到。
HIF-1α、VEGF 和 CD105 的表达水平无显著变化，以及 HIF-1α 和 GLUT-1 的表达模式表明，缺氧可能在 ACC 高级别转化过程中不起关键作用。尽管 HIF-1α 表达在 ACC 中很常见，但不能将其用作肿瘤侵袭性的标志物。
