Laboratory for the Analysis of Synthetic and Environmental Toxins, Centre for Research in Biopharmaceuticals and Biotechnology, Department of Biology, University of Ottawa, 20 Marie Curie Private, Ottawa, ON K1N 6N5, Canada.
Can J Physiol Pharmacol. 2012 Feb;90(2):167-74. doi: 10.1139/y11-121. Epub 2012 Feb 9.
Eight commercial grape seed products (GSPs) were assessed for their inhibition of the formation of advanced glycation end-products in vitro. All 8 commercial GSPs included in this study were potent inhibitors of advanced glycation end-product formation with IC(50) values ranging from 2.93 to 20.0 µg/mL. Total procyanidin content ranged from 60% to 73%. HPLC-DAD-ELSD results indicate that (+)-catechin, (-)-epicatechin, procyanidin B1, and procyanidin B2 were predominant and ubiquitously present in all the products under study, while gallic acid and procyanidin B4 were present in relatively minor amounts. The IC(50) values correlated with total phenolic content, and multiple regression analysis indicated that IC(50) is a linear function of the concentration of gallic acid and procyanidins B1, B2, and B4. Based on this study, GSPs have the potential to complement conventional diabetes medication toward disease management and prevention.
本研究评估了 8 种商业葡萄籽产品（GSP）对体外糖基化终产物形成的抑制作用。本研究中包含的 8 种商业 GSP 均为强效的糖基化终产物形成抑制剂，其 IC（50）值范围为 2.93 至 20.0μg/mL。总原花青素含量范围为 60%至 73%。HPLC-DAD-ELSD 结果表明，（+）-儿茶素、（-）-表儿茶素、原花青素 B1 和原花青素 B2 是所有研究产品中的主要成分且普遍存在，而没食子酸和原花青素 B4 的含量相对较少。IC（50）值与总酚含量相关，多元回归分析表明，IC（50）是没食子酸和原花青素 B1、B2、B4 浓度的线性函数。基于这项研究，GSP 有可能补充传统的糖尿病药物，用于疾病管理和预防。
