Institut für Medizinische Mikrobiologie und Immunologie der Universität Bonn, Sigmund-Freud-Straße 25, 53127 Bonn, Germany.
Diagn Microbiol Infect Dis. 2012 Mar;72(3):219-25. doi: 10.1016/j.diagmicrobio.2011.12.006.
Recently, members of the genus Tsukamurella have been implicated as important etiologic pathogens contributing to bloodstream and pulmonary infections in immunocompromised patients. Tsukamurella species share many features with other mycolic acid-containing genera of the order Actinomycetales and might therefore be misidentified as belonging to one of these genera. We developed a TaqMan-based real-time polymerase chain reaction assay for the rapid and specific detection of infections due to Tsukamurella species. The assay amplifies and detects a 157-bp segment of the 16S rRNA gene of Tsukamurella. The specificity of the assay was confirmed using a panel of DNAs from 12 Tsukamurella strains and 11 strains belonging to 8 phylogenetic closely related genera. The sensitive and specific nature of the assay provides a valuable tool for the early and precise diagnosis of Tsukamurella infections in clinical diagnostic laboratories.
最近，柄孢壳属的一些成员被认为是导致免疫功能低下患者血流感染和肺部感染的重要病原体。柄孢壳属与其他含分枝菌酸的放线菌目属有许多共同特征，因此可能被错误地鉴定为属于其中一个属。我们开发了一种基于 TaqMan 的实时聚合酶链反应检测方法，用于快速和特异性检测柄孢壳属引起的感染。该检测方法扩增和检测柄孢壳属 16S rRNA 基因的 157bp 片段。该检测方法的特异性通过使用来自 12 株柄孢壳属菌株和 11 株属于 8 个亲缘关系密切的属的菌株的 DNA 进行了验证。该检测方法的敏感性和特异性为临床诊断实验室中柄孢壳属感染的早期和准确诊断提供了有价值的工具。
