Division of Infectious Disease, Department of Pediatrics, Hyogo Prefectural Kobe Children hospital, Kobe City, 6500047, Hyogo, Japan.
Department of Inspection unit, Hyogo Prefectural Kobe Children hospital, Kobe City, Hyogo, Japan.
Ann Clin Microbiol Antimicrob. 2023 Nov 8;22(1):97. doi: 10.1186/s12941-023-00651-6.
Tsukamurella spp. are obligate aerobic, gram-positive, non-motile, and slightly acid-fast bacilli belonging to the Actinomycetes family. They share many characteristics with Nocardia, Rhodococcus, Gordonia, and the rapidly growing Mycobacterium species. Therefore, standard testing may misidentify Tsukamurella spp. as another species. Accurate and rapid diagnosis is critical for proper infection management, but identification of this bacterium is difficult in the standard laboratory setting.
A bloodstream infection caused by a gram-positive bacterium and related to a central venous catheter was identified in an immunocompromised 2-year-old girl. Tsukamurella tyrosinosolvens was identified by modified secA1 sequencing. Antibiotic treatment and removal of the central venous catheter resolved the infection. Inappropriate management of the catheter during an overnight stay outside of the hospital was considered as a possible source of infection.
SecA1 sequencing may be a useful diagnostic tool in the identification of T. tyrosinosolvens. Providing proper central venous catheter care instructions to patients, their families, and medical staff is important for infection prevention.
土拉弗朗西斯菌属是需氧、革兰阳性、非运动、略抗酸性的杆菌，属于放线菌科。它们与诺卡氏菌属、红球菌属、戈登氏菌属和快速生长分枝杆菌属有许多共同特征。因此，标准检测可能会将土拉弗朗西斯菌属误鉴定为其他物种。准确快速的诊断对于正确的感染管理至关重要，但在标准实验室环境中土拉弗朗西斯菌属的鉴定较为困难。
一名免疫功能低下的 2 岁女孩发生血流感染，与中心静脉导管相关。经改良 secA1 测序鉴定为土拉弗朗西斯菌酪氨酸亚种。通过抗生素治疗和中心静脉导管的移除，感染得到解决。考虑到在院外过夜期间对导管的不当处理可能是感染源。
secA1 测序可能是鉴定土拉弗朗西斯菌酪氨酸亚种的有用诊断工具。为患者、其家属和医务人员提供正确的中心静脉导管护理说明对于预防感染很重要。
