在用于生物燃料生产的分批培养过程中，淡水微藻 Scenedesmus sp. LX1 对可溶性藻产物 (SAP) 的积累特性。
Accumulation characteristics of soluble algal products (SAP) by a freshwater microalga Scenedesmus sp. LX1 during batch cultivation for biofuel production.
机构信息
Environmental Simulation and Pollution Control State Key Joint Laboratory, School of Environment, Tsinghua University, Beijing 100084, PR China.
出版信息
Bioresour Technol. 2012 Apr;110:184-9. doi: 10.1016/j.biortech.2011.11.023. Epub 2011 Nov 15.
Algae cultivation is the essential basis for microalgal biofuel production. Soluble algal products (SAP) are significant obstacle to large-scale, high-cell-density cultivation processes. SAP accumulation during batch cultivation of Scenedesmus sp. LX1 (a unique strain accumulating lipid substantially while growing fast under low-nutrient conditions) with different initial nitrogen concentrations (7.4-34.0mgNL(-1)) was investigated. The SAP content varied in the range of 3.4-17.4mgDOCL(-1) at stationary phase, with average yield per cell of 0.5-2.5pgDOCcell(-1). High SAP accumulation up to 15.2-17.4mgDOCL(-1) were observed with initial nitrogen above 20.2mgNL(-1). The maximum SAP production rate per unit culture volume (r(SAP)) was 2.6mgDOC(Ld)(-1) and that per cell (ν(SAP)) was 1.5pgDOC(celld)(-1). The r(SAP) increased with cell growth rate and decreased with cell density linearly. The SAP accumulation was majorly due to the release of growth-associated products.
藻培养是微藻生物燃料生产的基础。可溶性藻产物（SAP）是大规模、高细胞密度培养过程的重要障碍。在不同初始氮浓度（7.4-34.0mgNL（-1））下，对生长迅速且在低营养条件下大量积累脂质的独特 Scenedesmus sp. LX1 进行分批培养时，研究了 SAP 的积累情况。在静止期，SAP 含量在 3.4-17.4mgDOCL（-1）范围内变化，每个细胞的平均产量为 0.5-2.5pgDOCcell（-1）。初始氮浓度高于 20.2mgNL（-1）时，观察到高达 15.2-17.4mgDOCL（-1）的高 SAP 积累。单位培养体积的最大 SAP 生产速率（r（SAP））为 2.6mgDOC（Ld）（-1），每个细胞的 r（SAP）为 1.5pgDOC（celld）（-1）。r（SAP）随细胞生长速率线性增加，随细胞密度线性降低。SAP 的积累主要是由于生长相关产物的释放。
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