含牛磺酸眼内灌洗液对玻璃体切除术的神经保护作用。

Neuroprotective effects of a taurine-containing irrigation solution for vitrectomy.

机构信息

Department of Ophthalmology, University Eye Hospital Tübingen, Tübingen, Germany.

出版信息

Retina. 2012 Jul;32(7):1343-9. doi: 10.1097/IAE.0b013e318232c37c.

DOI:10.1097/IAE.0b013e318232c37c

PMID:22322280

Abstract

BACKGROUND

During pars plana vitrectomy, the retina is exposed to several iatrogenic risk factors, including excitotoxicity. A taurine-containing irrigation solution for pars plana vitrectomy (PURI PROTECT) has been developed and is claimed to have neuroprotective properties.

METHODS

Retinal ganglion cells (RGC-5) and retinal whole mounts were incubated in standard irrigation solution (SIS) and SIS supplemented with 3 mM taurine (SIS-taurine). Excitotoxicity was induced by the addition of 8, 10, and 12 mM or 250 μM glutamate. Cell viability and cell survival were assessed by the MTT test and Annexin-V/propidium iodide flow cytometry. Whole mounts were stained with the Live/Dead staining assay. Pars plana vitrectomy with SIS or SIS-taurine was performed in rabbits. Animals were followed-up by electroretinography.

RESULTS

RGC-5 incubated in SIS-taurine showed a 4.3-fold (P < 0.0005) better overall cell viability and an up to 8.5-fold (P < 0.05) increased cell survival under excitotoxic conditions compared with that incubated in SIS. Whole mounts incubated in SIS-taurine showed a 1.7-fold (P < 0.0005) and 1.6-fold (P < 0.0005) better cell survival under excitotoxic and nonexcitotoxic conditions, respectively. In the immediate postoperative period, b-wave amplitudes were significantly better in animals operated with SIS-taurine compared with control (P < 0.01).

CONCLUSION

A taurine-containing irrigation solution may protect retinal ganglion cells against excitotoxicity.

摘要

背景

在经平坦部玻璃体切除术（PPV）过程中，视网膜会暴露于多种医源性风险因素，包括兴奋性毒性。一种含有牛磺酸的 PPV 冲洗液（PURI PROTECT）已被开发出来，并被认为具有神经保护作用。

方法

视网膜神经节细胞（RGC-5）和视网膜全铺片在标准冲洗液（SIS）和补充 3 mM 牛磺酸的 SIS（SIS-牛磺酸）中孵育。通过加入 8、10 和 12 mM 或 250 μM 谷氨酸来诱导兴奋性毒性。通过 MTT 试验和 Annexin-V/碘化丙啶流式细胞术评估细胞活力和细胞存活率。使用 Live/Dead 染色测定法对全铺片进行染色。在兔子中进行 SIS 或 SIS-牛磺酸的 PPV。通过视网膜电图对动物进行随访。

结果

与在 SIS 中孵育的细胞相比，在 SIS-牛磺酸中孵育的 RGC-5 在兴奋性毒性条件下整体细胞活力提高了 4.3 倍（P < 0.0005），细胞存活率提高了 8.5 倍（P < 0.05）。在 SIS-牛磺酸中孵育的全铺片在兴奋性和非兴奋性条件下的细胞存活率分别提高了 1.7 倍（P < 0.0005）和 1.6 倍（P < 0.0005）。在术后即刻，与对照组相比，用 SIS-牛磺酸进行手术的动物的 b 波幅度明显更好（P < 0.01）。