Department of Surgery, Korea University College of Medicine, Seoul, Korea.
J Breast Cancer. 2011 Dec;14(4):269-75. doi: 10.4048/jbc.2011.14.4.269. Epub 2011 Dec 27.
Among more than 500 microRNAs, microRNA-21 (miR-21) is known to act as an oncogene. The aim of this study was to investigate the significance of miR-21 expression level in relation with clinicopathological factors and prognosis in breast cancer.
MicroRNA was extracted from cancer and normal breast tissue of 109 breast cancer patients who underwent surgery from 2002 to 2004 using the Taqman® MicroRNA Assay. The correlation between miR-21 expression and clinicopathologic features was analyzed and the significance of miR-21 as a prognostic factor and its relationship with survival was determined.
MiR-21 expression was higher in cancer tissues than in normal tissues (p<0.0001). High miR-21 expression was associated with mastectomy, larger tumor size, higher stage, higher grade, estrogen receptor (ER) negative, human epidermal growth factor receptor 2 (HER2) positive, HER2 positive breast cancer subtype, high Ki-67 expression, and death. On multivariate analysis, prognostic factors for overall survival were ER and miR-21. High miR-21 expression was significantly related to lower overall survival (p=0.031).
This study supports the role of miR-21 as an oncogene and a biomarker for breast cancer with its high expression in cancer tissues and its relationship with other prognostic factors and survival.
在超过 500 种 microRNAs 中，miR-21（microRNA-21）被认为是一种致癌基因。本研究旨在探讨 miR-21 表达水平与乳腺癌临床病理因素及预后的关系。
2002 年至 2004 年间，对 109 例接受手术的乳腺癌患者的癌组织和正常乳腺组织进行 microRNA 提取，采用 Taqman®MicroRNA 检测。分析 miR-21 表达与临床病理特征的相关性，确定 miR-21 作为预后因素的意义及其与生存的关系。
miR-21 在癌组织中的表达高于正常组织（p<0.0001）。高 miR-21 表达与乳房切除术、肿瘤较大、分期较高、分级较高、雌激素受体（ER）阴性、人类表皮生长因子受体 2（HER2）阳性、HER2 阳性乳腺癌亚型、高 Ki-67 表达和死亡有关。多因素分析显示，总生存率的预后因素为 ER 和 miR-21。高 miR-21 表达与总生存率显著降低相关（p=0.031）。
本研究支持 miR-21 作为致癌基因和乳腺癌生物标志物的作用，其在癌组织中的高表达及其与其他预后因素和生存的关系。
