Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China.
Bioresour Technol. 2012 Apr;110:534-8. doi: 10.1016/j.biortech.2011.12.015. Epub 2011 Dec 11.
Influence of dissolved oxygen (DO) levels (10%, 20%, 30% and 40%) on the glucosamine (GlcN) and N-acetylglucosamine (GlcNAc) production by recombinant Escherichia coli was investigated. It was found that the highest specific GlcN and GlcNAc production rates were obtained at different DO levels at different culture stages. Namely, the highest specific GlcN and GlcNAc production rates were obtained at 20% during 0-2h, 30% during 2-8h, 40% during 8-12h, and 30% during 12-18h. Accordingly, a step-wise DO control strategy was proposed, namely DO was controlled at 20% during 0-2h, 30% during 2-8h, 40% during 8-12h and 30% during 12-18h. With this DO control approach, the total production of GlcN and GlcNAc reached 72.89gL(-1), which was 1.37 times that without DO control (53.31gL(-1)). The developed step-wise DO control strategy may be useful for the industrial GlcN and GlcNAc production by recombinant E. coli.
考察了溶解氧（DO）水平（10%、20%、30%和 40%）对重组大肠杆菌生产氨基葡萄糖（GlcN）和 N-乙酰氨基葡萄糖（GlcNAc）的影响。结果发现，不同培养阶段不同 DO 水平下的 GlcN 和 GlcNAc 比生产速率最高。即在 0-2h 时 DO 控制在 20%、2-8h 时 DO 控制在 30%、8-12h 时 DO 控制在 40%、12-18h 时 DO 控制在 30%时，GlcN 和 GlcNAc 的比生产速率最高。因此，提出了一种逐步 DO 控制策略，即在 0-2h 时 DO 控制在 20%、2-8h 时 DO 控制在 30%、8-12h 时 DO 控制在 40%、12-18h 时 DO 控制在 30%。采用这种 DO 控制方法，GlcN 和 GlcNAc 的总产量达到 72.89gL(-1)，是不进行 DO 控制（53.31gL(-1)）时的 1.37 倍。所开发的逐步 DO 控制策略可能对重组大肠杆菌工业生产 GlcN 和 GlcNAc 有用。
