Nishizawa Yumie, Yabuki Takashi, Fukuda Eriko, Wakagi Takayoshi
Department of Biotechnology, The University of Tokyo, Japan.
FEBS Lett. 2005 Apr 25;579(11):2319-22. doi: 10.1016/j.febslet.2004.11.117.
A hyperthermophilic and aerobic crenarchaeon, Aeropyrum pernix K1, has two sets of genes possibly encoding 2-oxoacid:ferredoxin oxidoreductases. One is encoded in open reading frames (ORFs) ape2126 and ape2128, and the other in ORFs ape1473 and ape1472. The two sets of genes were expressed. The product enzymes, Ape2126/2128 and Ape1473/1472, showed optimal temperatures of 105 and over 110 degrees C, and optimal pHs of 8.5 and 9.0, respectively, using pyruvate as a substrate. Pyruvate, 2-oxobutyrate, and glyoxylate were the best substrates for both enzymes, and additionally Ape1473/1472 was able to act on 2-oxoglutarate, suggesting the enzyme operates in the TCA cycle.
嗜热需氧泉古菌——火球菌K1（Aeropyrum pernix K1）有两组可能编码2-氧代酸：铁氧化还原蛋白氧化还原酶的基因。一组由开放阅读框（ORF）ape2126和ape2128编码，另一组由ORF ape1473和ape1472编码。这两组基因都得到了表达。产物酶Ape2126/2128和Ape1473/1472，以丙酮酸为底物时，最适温度分别为105℃及超过110℃，最适pH分别为8.5和9.0。丙酮酸、2-氧代丁酸和乙醛酸是这两种酶的最佳底物，此外，Ape1473/1472还能作用于2-氧代戊二酸，这表明该酶在三羧酸循环中起作用。
