血清多氯联苯水平与哮喘和非哮喘日本儿童血液样本中白细胞介素 8 mRNA 表达的关联。
Association of serum levels of polychlorinated biphenyls with IL-8 mRNA expression in blood samples from asthmatic and non-asthmatic Japanese children.
机构信息
Department of Environmental Toxicology, University of California Davis, Davis, CA 95616, United States.
出版信息
Chemosphere. 2012 Jun;87(11):1228-34. doi: 10.1016/j.chemosphere.2012.01.022. Epub 2012 Feb 11.
BACKGROUND
One of the suggested health outcomes of PCB exposure is childhood asthma.
OBJECTIVES
This study was conducted to find health relevant biomarkers providing the molecular epidemiological evidence for the positive relationship between exposure to PCBs and childhood asthma.
METHODS
Blood samples from fifteen asthmatic children as well as an equal number of non-asthmatic children (average 2 year old) were collected, and were analyzed for PCBs and their select marker expression by using qRT-PCR.
RESULTS
Among biomarkers examined IL-8 expression was significantly correlated to serum levels of PCB #163+164 (P=0.022), #170 (P=0.046), #177 (P=0.022), #178 (P=0.022) and #180+193 (P=0.046) in a dose-dependent manner, which was found only among asthmatic children. In contrast, COX-2 correlations to individual congener levels were recognized only among control subjects, not among asthmatic subjects.
CONCLUSION
Serum concentrations of PCB#163+164, #170, #177, #178 and #180+193 correlate significantly with IL-8 mRNA expressions among asthmatic children.
背景
多氯联苯（PCBs）暴露被认为是导致儿童哮喘的一个健康影响因素。
目的
本研究旨在寻找与健康相关的生物标志物，为 PCBs 暴露与儿童哮喘之间的正相关关系提供分子流行病学证据。
方法
采集了 15 名哮喘儿童和数量相等的非哮喘儿童（平均年龄 2 岁）的血样，采用 qRT-PCR 方法分析 PCBs 及其选择标记物的表达。
结果
在所检查的生物标志物中，IL-8 的表达与血清中 PCB#163+164（P=0.022）、#170（P=0.046）、#177（P=0.022）、#178（P=0.022）和#180+193（P=0.046）的水平呈显著相关，且这种相关性仅存在于哮喘儿童中。相比之下，COX-2 与个体同系物水平的相关性仅在对照组中得到认可，而在哮喘组中未得到认可。
结论
在哮喘儿童中，血清中 PCB#163+164、#170、#177、#178 和#180+193 的浓度与 IL-8 mRNA 的表达显著相关。
Zotero 插件