血清中二缩水甘油醚双酚 A 特异性 IgG 与幼儿食物过敏的关系。

Association between bisphenol A diglycidyl ether-specific IgG in serum and food sensitization in young children.

机构信息

Department of Environmental Health, University of Occupational and Environmental Health, 1-1 Iseigaoka, Yahatanishi-ku, Kitakyusyu, Fukuoka Prefecture, 807-8555, Japan.

Department of Epidemiology and Preventive Medicine, Kagoshima University Graduate School of Medical and Dental Sciences, 8-35-1 Sakuragaoka, Kagoshima, 890-8520, Japan.

出版信息

Eur J Med Res. 2018 Dec 26;23(1):61. doi: 10.1186/s40001-018-0358-1.

DOI:10.1186/s40001-018-0358-1

PMID:30587237

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6306001/

Abstract

BACKGROUND

Recent studies have reported that endocrine-disrupting compound (EDC) exposure is related to food sensitization. Bisphenol A diglycidyl ether (BADGE) is one of the most widespread EDCs and its biological effects are considered to be greater on children than on adults. This study investigated the relationship between serum BADGE-specific immunoglobulin G (IgG) concentrations and food sensitization in young children by measuring food-specific IgE levels.

METHODS

In total, 98 young children (59 boys and 39 girls; median age: 7 months; 25th and 75th percentile ages: 6 and 8 months, respectively) were enrolled. Blood samples were collected twice from all children (median sampling interval: 6 months; 25th and 75th percentile: 5 and 7 months). Food sensitization was evaluated based on food-specific IgE titers (egg white, milk, and wheat), which were determined using the capsulated hydrophilic carrier polymer-radioallergosorbent test. Furthermore, a dot-blotting assay for BADGE-specific IgG and quantitative reverse-transcriptase PCR for IL-6, IL-8, IL-10, and COX-2 mRNA expression were conducted.

RESULTS

BADGE-specific IgG was detected in 20% of study subjects. A significant association was observed between the presence of BADGE-specific IgG and elevated wheat-specific IgE levels (OR = 3.56; 95% CI 1.13-11.2; P = 0.031). This relationship was particularly strong in girls (OR = 9.46; 95% CI 1.01-89.0; P = 0.049). A slight but non-significant association was noted between the presence of BADGE-specific IgG and elevated milk-specific IgE levels (OR = 2.77; 95% CI 0.93-8.22; P = 0.067). The expression of IL-6 mRNA among children with BADGE-specific IgG tended to increase, along with wheat-specific IgE levels.

CONCLUSION

BADGE exposure might enhance food sensitization in early childhood. Therefore, this should be strictly regulated, especially in younger children.

摘要

背景

最近的研究报告称，内分泌干扰化合物（EDC）暴露与食物过敏有关。双酚 A 二缩水甘油醚（BADGE）是最广泛的 EDC 之一，其生物效应被认为对儿童比对成人更大。本研究通过测量食物特异性 IgE 水平，调查了血清 BADGE 特异性 IgG 浓度与幼儿食物过敏之间的关系。

方法

共纳入 98 名幼儿（59 名男孩和 39 名女孩；中位年龄：7 个月；25 百分位数和 75 百分位数年龄：6 和 8 个月）。所有儿童均采集两次血样（中位采样间隔：6 个月；25 百分位数和 75 百分位数：5 和 7 个月）。基于食物特异性 IgE 滴度（蛋清、牛奶和小麦）评估食物过敏，使用囊泡亲水性载体聚合物放射过敏原吸附试验进行检测。此外，进行了 BADGE 特异性 IgG 的斑点印迹分析和 IL-6、IL-8、IL-10 和 COX-2 mRNA 表达的定量逆转录酶 PCR。

结果

在 20%的研究对象中检测到 BADGE 特异性 IgG。BADGE 特异性 IgG 的存在与小麦特异性 IgE 水平升高显著相关（OR=3.56；95%CI 1.13-11.2；P=0.031）。这种关系在女孩中尤为明显（OR=9.46；95%CI 1.01-89.0；P=0.049）。BADGE 特异性 IgG 的存在与牛奶特异性 IgE 水平升高略有但无统计学意义的关联（OR=2.77；95%CI 0.93-8.22；P=0.067）。在具有 BADGE 特异性 IgG 的儿童中，IL-6 mRNA 的表达随着小麦特异性 IgE 水平的升高而趋于增加。