Department of Chemical and Biomolecular Engineering, Yonsei University, 134 Shinchon-dong, Seodaemun-gu, Seoul 120-749, Republic of Korea.
Int J Pharm. 2012 May 10;427(2):417-25. doi: 10.1016/j.ijpharm.2012.01.050. Epub 2012 Feb 2.
Cross-linked alginate beads containing adenovirus (Ad) were successfully fabricated using an electrospraying method to achieve the protection and release of Ad in a controlled manner. An aqueous alginate solution containing Ad was electrosprayed into an aqueous phase containing a cross-linking agent (calcium chloride) at different process variables (voltages, alginate concentrations, and flow rates). Alginate beads containing Ad were used for transduction of U343 glioma cells and the transduction efficiency of the alginate beads was measured by quantification of gene expression using a fluorescence-activated cell sorter at different time points. In vitro results of gene expression revealed that the Ad encapsulated in the alginate beads with 0.5 wt% of alginate concentration exhibited a high activity for a long period (over 7 days) and was released in a sustained manner from the alginate beads. The Ad-encapsulating alginate beads could be promising materials for local delivery of Ad at a high concentration into target sites.
采用静电纺丝法成功制备了载腺病毒（Ad）的交联海藻酸钠珠，以实现 Ad 的保护和控制释放。将含有 Ad 的海藻酸钠溶液以不同的工艺变量（电压、海藻酸钠浓度和流速）静电纺丝到含有交联剂（氯化钙）的水相中。含有 Ad 的海藻酸钠珠用于转导 U343 神经胶质瘤细胞，并通过在不同时间点使用荧光激活细胞分选器定量基因表达来测量海藻酸钠珠的转导效率。体外基因表达结果表明，在 0.5wt%的海藻酸钠浓度下包封的 Ad 在较长时间内（超过 7 天）具有高活性，并以持续的方式从海藻酸钠珠中释放。载腺病毒的海藻酸钠珠有望成为在靶部位高浓度局部递送腺病毒的有前途的材料。
