利用激光散斑对比成像评估系统微血管内皮功能。
Evaluation of systemic microvascular endothelial function using laser speckle contrast imaging.
机构信息
National Institute of Cardiology (INC), Rio de Janeiro, Brazil.
出版信息
Microvasc Res. 2012 May;83(3):376-9. doi: 10.1016/j.mvr.2012.01.004. Epub 2012 Jan 31.
OBJECTIVE
The aim of this study was to compare cutaneous microvascular function in young healthy subjects (n=50) with that of cardiometabolic diseased patients (n=50) using laser speckle contrast imaging (LSCI) coupled with transdermal iontophoretic delivery of acetylcholine (ACh) and post-occlusive reactive hyperemia (PORH).
METHODS
Cutaneous blood flow was assessed in the forearm using LSCI at rest, during PORH and during iontophoresis of ACh with increasing anodal currents of 30, 60, 90, 120, 150 and 180 μA during 10-second intervals spaced 1 min apart.
RESULTS
Endothelium-dependent skin microvascular vasodilator responses induced by both ACh and PORH were significantly reduced in cardiometabolic diseased patients compared to healthy subjects. Vasodilator responses induced by ACh were significantly higher in young women than in young men. Iontophoresis charges up to 1.5 mC do not induce nonspecific effects on skin microvascular flux.
CONCLUSION
LSCI appears to be a promising noninvasive technique for evaluating systemic microvascular endothelial function.
目的
本研究旨在使用激光散斑对比成像（LSCI）联合经皮离子电渗递乙酰胆碱（ACh）和闭塞后反应性充血（PORH），比较年轻健康受试者（n=50）和合并心血管代谢疾病患者（n=50）的皮肤微血管功能。
方法
使用 LSCI 在休息、PORH 和 30、60、90、120、150 和 180 μA 递增阳极电流下间隔 1 分钟进行 10 秒离子电渗递 ACh 时，在前臂评估皮肤血流。
结果
与健康受试者相比，合并心血管代谢疾病患者的 ACh 和 PORH 诱导的内皮依赖性皮肤微血管舒张反应明显降低。ACh 诱导的血管舒张反应在年轻女性中明显高于年轻男性。高达 1.5 mC 的离子电渗递电荷量不会对皮肤微血管通量产生非特异性影响。
结论
LSCI 似乎是一种有前途的评估系统性微血管内皮功能的非侵入性技术。
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