State Key Laboratory of Rice Biology and Ministry of Agriculture Key Laboratory of Agricultural Entomology, Institute of Insect Sciences, Zhejiang University, Hangzhou, China.
J Insect Physiol. 2012 Mar;58(3):420-6. doi: 10.1016/j.jinsphys.2012.01.012. Epub 2012 Feb 3.
In this study, we cloned full-length EcR cDNAs from the small brown planthopper Laodelphgax striatellus, the brown planthopper Nilaparvata lugens and the white back planthopper Sogatella furciferas. This is the first reporting of EcRs from either L. striatellus or S. furciferas. The deduced amino acid sequences of the EcRs show high levels of similarity to each other. The highest transcriptional level of the EcR gene was detected in the mid-fifth instar nymphs of N. lugens. Silencing of EcR expression by in vivo RNAi generated phenotypic defects in molting and resulted in lethality in most of the treated nymphs. Intriguingly, apparent wing defects in morphogenesis and melanization occurred during EcR knockdown in L. striatellus nymphs.
在这项研究中，我们从褐飞虱、白背飞虱和斜纹夜蛾中克隆了全长的 EcR cDNA。这是首次报道褐飞虱或斜纹夜蛾的 EcRs。EcR 的推导氨基酸序列彼此之间具有高度的相似性。在褐飞虱的第五龄中期若虫中，EcR 基因的转录水平最高。通过体内 RNAi 沉默 EcR 表达会导致蜕皮表型缺陷，并导致大多数处理过的若虫死亡。有趣的是，在褐飞虱若虫的 EcR 敲低过程中，出现了明显的翅膀形态和黑化缺陷。
