Jain Ritesh G, Robinson Karl E, Fletcher Stephen J, Mitter Neena
Queensland Alliance for Agriculture and Food Innovation, Centre for Horticultural Sciences, The University of Queensland, Brisbane 4072, Queensland, Australia.
Insects. 2020 Aug 20;11(9):557. doi: 10.3390/insects11090557.
RNA interference (RNAi) is a powerful approach for sequence-specific gene silencing, displaying tremendous potential for functional genomics studies in hemipteran insects. Exploiting RNAi allows the biological roles of critical genes to be defined and aids the development of RNAi-based biopesticides. In this review, we provide context to the rapidly expanding field of RNAi-based functional genomics studies in hemipteran insects. We highlight the most widely used RNAi delivery strategies, including microinjection, oral ingestion and topical application. Additionally, we discuss the key variables affecting RNAi efficacy in hemipteran insects, including insect life-stage, gene selection, the presence of nucleases, and the role of core RNAi machinery. In conclusion, we summarise the application of RNAi in functional genomics studies in Hemiptera, focusing on genes involved in reproduction, behaviour, metabolism, immunity and chemical resistance across 33 species belonging to 14 families.
RNA干扰（RNAi）是一种强大的序列特异性基因沉默方法，在半翅目昆虫的功能基因组学研究中显示出巨大潜力。利用RNAi可以确定关键基因的生物学作用，并有助于开发基于RNAi的生物杀虫剂。在这篇综述中，我们为半翅目昆虫中迅速发展的基于RNAi的功能基因组学研究领域提供背景信息。我们重点介绍了最广泛使用的RNAi递送策略，包括显微注射、口服摄入和局部应用。此外，我们讨论了影响半翅目昆虫RNAi效率的关键变量，包括昆虫的生命阶段、基因选择、核酸酶的存在以及核心RNAi机制的作用。总之，我们总结了RNAi在半翅目功能基因组学研究中的应用，重点关注了14个科33个物种中与生殖、行为、代谢、免疫和化学抗性相关的基因。
