Lenardon Megan D, Nantel André
School of Medical Sciences, University of Aberdeen, Aberdeen, UK.
Methods Mol Biol. 2012;845:41-9. doi: 10.1007/978-1-61779-539-8_3.
Techniques used to generate mutants in Candida albicans commonly result in additional and undesired genetic rearrangements. Detection of aneuploidy is, therefore, an important step forward in the quality control of mutant phenotypes. In this chapter, we describe how to extract genomic DNA and perform a quantitative multiplex PCR to compare the karyotype of any mutant strain to that of its parent and allow the detection of any unwanted aneuploidy.
用于在白色念珠菌中产生突变体的技术通常会导致额外的、不期望的基因重排。因此，检测非整倍体是突变体表型质量控制向前迈出的重要一步。在本章中，我们描述了如何提取基因组DNA并进行定量多重PCR，以比较任何突变菌株与其亲本的核型，并检测任何不想要的非整倍体。
