Tait E, Simon M C, King S, Brown A J, Gow N A, Shaw D J
Department of Molecular and Cell Biology, University of Aberdeen Medical School, Scotland.
Fungal Genet Biol. 1997 Jun;21(3):308-14. doi: 10.1006/fgbi.1997.0983.
A new project to map the genome of the pathogenic fungus, Candida albicans, has been started. The entire genome was cloned as 5088 cosmids, stored in individual microtiter plate wells. DNA was prepared and fingerprinted using restriction digestion, fluorescent labeling, and analysis on an ABI sequencer. These data are being used to construct contigs of the genome. Simultaneously, a DNA pooling system has been set up, suitable for PCR-based isolation of cosmids containing any known gene. Ultimately, these approaches will lead to the creation of a physically based map of the C. albicans genome, providing the means to localize precisely all the genes, act as a substrate for genome sequencing projects, and provide probes for future studies of genome rearrangement and comparative genomics.
一项绘制致病性真菌白色念珠菌基因组图谱的新项目已经启动。整个基因组被克隆为5088个黏粒，储存在单独的微量滴定板孔中。制备DNA并使用限制性消化、荧光标记以及在ABI测序仪上进行分析来生成指纹图谱。这些数据正用于构建基因组的重叠群。同时，已经建立了一个DNA池系统，适用于基于PCR分离含有任何已知基因的黏粒。最终，这些方法将促成构建白色念珠菌基因组的物理图谱，提供精确定位所有基因的手段，作为基因组测序项目的底物，并为未来的基因组重排和比较基因组学研究提供探针。
